Bulk Human IgG2 (IB2) Isotype Control

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Only %1 left
SKU
ICH2255
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$390.00 - $2,549.00

Bulk Human IgG2 (IB2) Isotype Control

Endotoxin
<1.0 EU/mg as determined by the LAL method
Purity
≥95% monomer by analytical SEC, >95% by SDS Page
Species Reactivity
Human
Target
IgG2
Concentration
≥ 5.0 mg/ml
Isotype
Human IgG2
Host
Human HEK-293
Aggregation
Aggregation level ≤ 5%
Buffer
ICH3001-100ml ICH3002-100ml ICH3003-100ml
Uniprot
P01859
Shipping Conditions
Blue ice
Background
Clone IB2 is a human IgG2 isotype control that delivers a subclass-matched negative reference for experiments employing IgG2-format test antibodies. Because IB2 carries no relevant specificity, it establishes the background and gating baseline needed to separate true antigen engagement from scaffold effects in flow cytometry and functional assays. Featuring the native IgG2 framework, control clone IB2 reproduces the Fc behaviour of the test antibody without contributing specific binding. It is supplied in a low-endotoxin format suitable for in vivo use and manufactured in scalable milligram to gram quantities for bulk and high-throughput laboratories. The reagent is non-therapeutic and for research use only.
Clone
IB2
Specificity
This Human IgG2 isotype control recombinant antibody has been tested against selected species' cells and tissues to assure minimal cross reactivity. Furthermore, this low endotoxin Human IgG2 antibody is suitable for in vivo applications and each lot is IMPACT I certified.
Purification Method
This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Storage
This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
Applications
Flow Cytometry, Functional Assays
Use
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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