Anti-Mouse IFNAR1 (MAR1-5A3) In Vivo Antibody - Low Endotoxin

Mouse

IFNAR1

≤ 2.0 mg/ml

Mouse IgG1

Mouse

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

Blocking: Clone MAR1-5A3 has a short half-life, basically because every cell expresses the IFNAR1 receptor and the receptor recycles very rapidly. And, if you want to block function in vivo, you need to be sure that all of the receptors are blocked continually in all compartments. Therefore, you need a large in vivo loading dose (2.5 mg/mouse) to saturate all the binding sites in vivo and then maintain a high enough level to keep them saturated. For in vivo blocking studies we recommend give a loading dose of 2.5 mg/mouse and follow with a weekly dose of 0.5 mg/mouse. The half-live following a 2.5 mg loading dose is about 5 days. [However, if you only inject a low dose of 250 micrograms, then the half life is 1.5 days – because you haven’t saturated the mouse]. Each investigator should determine their own optimal working dilution for specific applications.

Immunoprecipitation, Western Blot, Blocking, Functional Assays, Flow Cytometry, ELISA

anti-IFNAR1 In Vivo Antibody - Low Endotoxin (MAR1-5A3) is stable for at least four (4) weeks when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at -80°C in a manual defrost freezer.

0.01 M phosphate buffered saline (PBS) pH 7.2 - 7.4, 150 mM NaCl with no carrier protein, potassium or preservatives added.

This monoclonal antibody was purified using Protein A

Anti-IFNAR1 In Vivo Antibody - Ultra Low Endotoxin (MAR1-5A3) recognizes the extracellular domain of the IFNAR1 subunit of the mouse IFN-alpha / beta receptor.

Blue ice

IFNAR1 is a subunit of the type I interferon receptor required for IFN-alpha and IFN-beta signalling, antiviral defence and immune regulation. Clone MAR1-5A3 is a mouse IgG1 monoclonal antibody to mouse IFNAR1, validated for immunoprecipitation, Western blot, blocking, functional assays, flow cytometry and ELISA. Anti-mouse IFNAR1 MAR1-5A3 is supplied in a low-endotoxin, in-vivo-suitable format and manufactured in scalable milligram (mg) to gram (g) quantities for bulk laboratories. As a non-therapeutic, research-use-only (RUO) reagent, MAR1-5A3 provides reproducible blockade and detection of type I interferon signalling in the mouse.

Interferon alpha/beta receptor 1, IFN-R-1, IFN-alpha/beta receptor 1, Type I interferon receptor 1, Ifar, Ifnar

MAR1-5A3

P33896

ICH3001-100ml ICH3002-100ml ICH3003-100ml

IFNAR1 and IFNAR2 are coexpressed on nearly all cells.

This antibody was produced by In vivo genetic immunization of IFNAR1 knockout mice with a plasmid encoding the extracellular domain of murine IFNAR1.

Aggregation level ≤ 5%

Anti-IFNAR1 In Vivo Antibody - Ultra Low Endotoxin [MAR1-5A3] (ICH1122UL)