Universal Recombinant Mouse IgG Antibody Mix

No Human, Mouse, or Rat Antigens

Hen Egg Lysozyme

Mouse IgG1, IgG2a, IgG2b, and IgG3 kappa

Human IgG1 Fc plus hinge region (ICH2267), Human IgG4 Fc plus hinge region (ICH2268), Human IgG2 Fc plus hinge region (ICH2269)

ICH3002

Blue ice

This product is a recombinant mouse IgG antibody mix based on the well-characterised HyHEL-10 clone, engineered on multiple mouse heavy-chain backbones so that IgG1, IgG2a, IgG2b, and IgG3 kappa isotypes are all represented. Because it is directed against Hen Egg Lysozyme, a protein with no orthologue in human, mouse, or rat, it recognises no mammalian self-antigen and therefore serves as a matched, non-targeting reference reagent. This makes it ideally suited to functional and in-vitro research as an isotype control across ELISA, flow cytometry, immunoblotting, immunoprecipitation, immunocytochemistry, and immunofluorescence, allowing investigators to distinguish antigen-specific signal from Fc-receptor engagement and non-specific binding. Being recombinant gives lot-to-lot consistency and a defined sequence, and the reagent is offered as a low-endotoxin, bulk-scale, research-use-only product suitable for demanding cell-based and multiplexed assays. The multi-isotype composition lets a single reagent supply isotype-matched controls for a panel of primary antibodies of differing subclass. Hen Egg Lysozyme (HEL) is a small (~14.3 kDa, 129-residue) enzyme from chicken egg white and the archetypal c-type (chicken-type) lysozyme. It hydrolyses the beta-1,4 glycosidic bond between N-acetylmuramic acid and N-acetylglucosamine in bacterial peptidoglycan, contributing to innate antimicrobial defence. HEL has been a foundational model protein in structural biology, protein folding, and immunology: it was among the first enzymes solved by X-ray crystallography and its compact, disulphide-stabilised fold is exceptionally well defined. In immunology, HEL is a canonical model antigen and the HyHEL series of anti-lysozyme antibodies are textbook systems for studying antibody-antigen recognition, affinity maturation, and epitope mapping. Critically for reagent design, HEL has no counterpart in human, mouse, or rat proteomes, so antibodies against it do not cross-react with mammalian tissue, which is precisely why anti-HEL clones are widely used as non-targeting negative and isotype controls.

Recombinant Mouse IgG Blocking Solution; Alternative of Serum-derived Mouse IgGs, HyHEL 10, HyHEL10

HyHEL-10

HEL

The recombinant mouse IgG antibodies were affinity-purified from supernatant of mammalian cells cultured in a chemically defined medium by affinity-chromatography using protein A and G.

0.2 uM filtered solution, pH 7.4, no stabilizers or preservatives.

Stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –20°C or –80°C. Avoid Repeated Freeze Thaw Cycles.

ELISA, flow cytometry, immunoblotting, immunoprecipitation, immunocytochemistry, immunofluorescence, and isotype controls.

Products are for research use only. Not for use in diagnostic or therapeutic procedures.