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Anti-CD86 (GL1) In Vivo Antibody - Low Endotoxin

Name: Anti-CD86 (GL1) In Vivo Antibody - Low Endotoxin
SKU: ICH1038
Price: 400 USD
Availability: InStock

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SKU

ICH1038

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$400.00 - $3,780.00

Anti-CD86 (GL1) In Vivo Antibody - Low Endotoxin

Endotoxin

≤ 1.0 EU/mg as determined by the LAL method

Purity

>95% by SDS-PAGE and HPLC

Species Reactivity

Mouse

Target

CD86

Concentration

1.0 mg/ml

Isotype

Rat IgG2a Kappa

Host

Rat

Use

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

Application Notes

Each investigator should determine their own optimal working dilution for specific applications.

Applications

Flow Cytometry, Immunoprecipitation, IHC (Frozen), Immunocytochemistry, Blocking, Immunofluorescence, Western Blot, CyTOF

Storage

anti-CD86 In Vivo Antibody - Low Endotoxin (GL1) is stable when stored at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Formulation

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.

Purification Method

This monoclonal antibody was purified using Protein G

Specificity

Anti-CD86 In Vivo Antibody - Ultra Low Endotoxin (GL1) recognizes an epitope on Mouse CD86

Shipping Conditions

Blue ice

Background

CD86 (B7-2) is a co-stimulatory molecule on activated antigen-presenting cells that binds CD28 and CTLA-4 to fine-tune T-cell activation. Clone GL1 is a rat IgG2a kappa monoclonal antibody recognising mouse CD86, validated for flow cytometry, immunoprecipitation, frozen-section IHC, immunocytochemistry, blocking, immunofluorescence, Western blot and CyTOF. Designed for in vivo use, low endotoxin GL1 is manufactured in scalable milligram (mg) to gram (g) quantities for high-throughput and heavy-usage laboratories. As a non-therapeutic reagent for research use only (RUO), anti-CD86 clone GL1 delivers reliable, reproducible results across murine immunology applications.

Other Names

Activation B7-2 antigen, Early T-cell costimulatory molecule 1, ETC-1

Clone

GL1

Uniprot

P42082

Buffer

ICH3001-100ml ICH3002-100ml ICH3003-100ml

Antigen Distribution

B cells and T cells (upregulated upon activation), macrophages, dendritic cells, and astrocytes

Immunogen

LPS-activated CBA/Ca mouse splenic B cells