Ichor-Detect™ Goat anti-Mouse IgG (H&L) F(ab')2 Secondary Antibody (APC), Cross-Adsorbed

Mouse

IgG (H&L)

0.5 mg/ml

Goat

Purified mouse IgG containing all isotypes.

Ambient

Multicolour flow cytometry depends on bright, specific secondary reagents, which is exactly what this IchorDetect goat anti-mouse IgG (H&L) F(ab')2 secondary antibody delivers. Conjugated to allophycocyanin (APC) and cross-adsorbed against other species' immunoglobulins, it provides strong far-red fluorescence with reduced background. The F(ab')2 format removes the Fc portion to limit nonspecific Fc-receptor binding, sharpening detection of mouse primary antibodies. It is a non-therapeutic reagent for research use only, supplied in a low-endotoxin format suitable for in vivo use and produced in scalable milligram (mg) to gram (g) quantities to meet the demands of bulk and high-throughput cytometry laboratories.

Fab2 Secondary Antibody APC

Goat F(ab')2 Anti-Mouse IgG (H&L) recognizes Mouse IgG (H&L). This secondary antibody was purified using antigen affinity chromatography and adsorbed against serum proteins and immunoglobulins to minimize species cross-reactivity.

This secondary antibody is lyophilized from 0.01M phosphate buffered saline (PBS) pH 7.6 containing 0.25M NaCl, 15 mg/ml IgG free BSA and 0.05% sodium azide as a preservative.

This lyophilized secondary antibody conjugate should be stored desiccated at 2-8°C. The reconstituted Allophycocyanin (APC) conjugate should be stored at 2-8°C. Do Not Freeze.

It is recommended that working dilutions be prepared fresh daily. A working dilution of 1:50 - 1:200 is recommended for most applications. Excitation Laser: Red Laser (633 nm)

Products are for research use only. Not for use in diagnostic or therapeutic procedures.