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Bulk Human IgG4 (IB4) Isotype Control S228P - Extremely Low Endotoxin
$937.00 - $6,503.00
Bulk Human IgG4 (IB4) Isotype Control S228P - Extremely Low Endotoxin
Endotoxin
<1.0 EU/mg as determined by the LAL method
Purity
>95% by SDS-PAGE
Species Reactivity
Human
Target
IgG4
Concentration
5mg/ml
Isotype
Human IgG4 S228P Kappa
Host
CHO
Aggregation
Aggregation level ≤ 5%
Immunogen
Hen Egg Lysozyme (HEL)
Buffer
ICH3001-100ml
ICH3002-100ml
ICH3003-100ml
Uniprot
P01861
Shipping Conditions
Blue ice
Background
Clone IB4 with the S228P hinge stabilisation is a human IgG4 isotype control supplied at extremely low endotoxin levels, making it a clean negative control for sensitive in vivo and functional immunology work. The S228P change prevents IgG4 Fab-arm exchange, while the absence of relevant specificity lets IB4 establish the background and gating reference that distinguishes genuine binding from scaffold signal in flow cytometry and functional assays. Control clone IB4 S228P is produced in scalable milligram to gram quantities and formulated for in vivo use. It is a research-use-only, non-therapeutic reagent suited to bulk and high-throughput laboratories.
Clone
IB4
Specificity
This Human IgG4 isotype control recombinant antibody has been tested against selected species' cells and tissues to assure minimal cross reactivity. Furthermore, this low endotoxin Human IgG4 antibody is suitable for in vivo applications. This Human IgG4 isotype control contains the S228P mutation
Purification Method
This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Fc Modification
S228P
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Storage
This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
Applications
Flow Cytometry, Functional Assays
Application Notes
Each investigator should determine their own optimal working dilution for specific applications.
Use
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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