Human CD32b Protein - Biotin

Human

Human CD32b Protein

Human embryonic kidney (HEK) 293 cells.

P31994

Ambient

As the dominant inhibitory Fcγ receptor, CD32b (FcγRIIb) tempers B-cell and myeloid signalling and governs how antibodies engage the immune system. Here the human CD32b extracellular domain is offered as a biotinylated recombinant protein from HEK 293 cells, ready for streptavidin capture in SPR, BLI and ELISA formats where oriented immobilization improves kinetic measurements of Fc binding. This is a non-therapeutic reagent for research use only. Manufactured to in vivo grade standards with ultra-low endotoxin and available in scalable milligram (mg) to gram (g) batches, it is built for heavy-usage and high-throughput laboratories studying Fc receptor engagement and antibody engineering.

Human Fc gamma RIIb, CD32B, FCGR2B, FCGRIIB, FCR2B, FCRIIB, IGFR2B, IGFRIIB

The sequence of the extracellular domain of human CD32b (Ala 46-Pro 217) was fused with a C-terminal tag consisting of the AVI tag, TEV protease recognition sequence and a 10-His tag.

Lyophilized from sterile PBS, pH 7.4. No preservatives or cryoprotectants have been added. To obtain a final concentration of 1 mg/ml reconstitute 0.25 mg vials with 0.25 ml water and 1.0 mg vials with 1.0 ml water.

Lyophilized proteins are stable at ambient temperature for at least 2 weeks. If the protein is not to be used immediately then the protein should be stored in lyophilized form at -20 °C for up 12 months. Once the protein has been reconstituted we recommen

SPR, BLI, ELISA

Products are for research use only. Not for use in diagnostic or therapeutic procedures.