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D Luciferin
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Bulk recombinant human PD1 protein
Expression System:
HEK 293 cells
Active:
Yes: measured by its ability to bind PDL1 at a sub-micromolar range in a functional ELISA.
Tags:
His tagged C-Terminus
Other Names:
Human PD1 Fusion Protein, PD1 His tag protein, PD1 protein his tagged, low endotoxin PD1 recombinant protein, Programmed death-1, CD279, PDCD1, Programmed Death-1 Receptor
Specificity:
Recombinant human PD1-His fusion protein is a HEK 293 protein fragment amino acids 25-167 fused with a polyhistidine tag at the C-terminus.
Formulation:
0.2 μM filtered solution of 30 mM HEPES, 150 mM NaCl, pH 7.
Purity:
>95% by SDS-PAGE and HPLC
Endotoxin:
<1.0 EU/mg as determined by the LAL method
Storage:
Bulk PD1 protein is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Applications:
HPLC, SDS-PAGE, Functional Studies, ELISA
Application Notes:
Each investigator should determine their own optimal working dilution for specific applications.
Use:
Products are for research use only.
| Concentration | 12.76mg/ml |
|---|---|
| Buffer | ICH3002 |
| Formulation | Powder |
| Purity | >99% |
| Endotoxin | <0.05 EU/mg as determined by the LAL method |
| Storage | -20°C. |
| Applications | Bioluminescence Imaging |
| Application Notes | Use at a working dilution of 150 ug/ml for in vitro bioluminescence assay, use at 10 ul/g of body weight for in vivo imaging |
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