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Bulk Anti-Human CD25 (7G7B6) Antibody
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Bulk anti-human CD25 antibody (7G7B6)
Bio X Cell:ICH1163 is up to 37% cheaper for industry than the equivalent product BE0014 from Bio X Cell. Product Benefits:
ichorbio's bulk anti-human CD25 (IL-2Rα) antibody (7G7B6) is manufactured in a cGMP compliant, ISO Quality Standard 9001:2015 facility. ichorbio's low endotoxin antibodies have half the endotoxin of comparable antibodies from Bio X Cell at less than 1.0 EU/mg. If ichorbio's low endotoxin antibodies are not low enough we also offer ultra low endotoxin antibodies which have even less endotoxin (<0.5EU/mg) at an even higher purity (98% versus 95%).
Target:CD25
Clone:7G7B6
Size:ichorbio's 7G7B6 in vivo antibody is available in the following bulk sizes: 1mg, 5mg, 25mg, 50mg and 100mg ichorbio regularly manufactures multi-gram amounts of our anti-CD25 7G7B6 clone - please contact us for pricing.
Isotype:Mouse IgG2a
Other Names:IL2RA, Interleukin-2 receptor subunit alpha, TAC, p55
Uniprot: Host:Mouse
Species Reactivity:Human
Specificity:Clone 7G7B6 reacts with human CD25
Purification Method:This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Antigen Distribution:IL-2Rα is expressed on activated mature T and B lymphocytes, during early stages of thymocytes development, pre-B cells, and in activated CD4+ memory T-lymphocytes
Background:CD25 (IL-2Rα) is the 55 kDa subunit of the interleukin 2 receptor alpha chain which has been shown to play roles in lymphocyte differentiation, activation, and proliferation. IL-2Rα is a transmembrane glycoprotein comprised of a heavily glycosylated extracellular domain, a transmembrane region and a short cytoplasmic tail. IL-2Rα heterodimerizes with IL-2Rβ (CD122) and IL-2Rγ (CD132) and forms the IL-2 receptor complex. IL-2Rα by itself binds IL-2 with relatively low affinity, while the IL-2 receptor complex binds IL-2 with high affinity. IL-2Rα mutations cause severe immunodeficiency.
Immunogen:Recombinant human peripheral blood mononuclear cells (PBMCs)
Concentration:1.0 mg/ml
Formulation:This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (PBS) pH 7.2 - 7.4, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and azide free
Purity:>95% by SDS-PAGE and HPLC
>98% by SDS-PAGE and HPLC
Endotoxin:≤ 1.0 EU/mg as determined by the LAL method
≤ 0.75 EU/mg as determined by the LAL method
Aggregation:Aggregation level ≤ 5%
Aggregation level ≤ 1%
IMPACT Pathogen Test:We use the IMPACT test generated by IDEXX Laboratories to guarantee our Ultra Low Endotoxin antibodies are pathogen free. Our mouse antibodies are tested for: Mycoplasma spp. Mycoplasma pulmonis Sendai virus Mouse hepatitis virus Pneumonia virus of mice Minute virus of mice Mouse parvovirus (MPV1-5) Theiler’s murine encephalomyelitis virus Murine norovirus Reovirus 3 Mouse rotavirus Ectromelia virus Lymphocytic choriomeningitis virus Polyoma virus Lactate dehydrogenase-elevating virus Mouse adenovirus (MAD1, MAD2) Mouse cytomegalovirus K virus Mouse thymic virus Hantaan virus Corynebacterium bovis Corynebacterium spp. (HAC2)
Storage:This antibody is stable for at least 4 weeks when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 20°C or -80°C. Avoid repeated freeze thaw cycles.
Applications:ELISA, Flow Cytometry, Immunofluorescence Staining, Immunoprecipitation, Western Blotting
Application Notes:Each investigator should determine their own optimal working dilution for specific applications.
Use:Products are for research use only.
| Concentration | 1mg/ml |
|---|---|
| Isotype | Mouse IgG2a |
| Host | Mouse |
| Use | Products are for research use only. Not for use in diagnostic or therapeutic procedures. |
| Application Notes | Each investigator should determine their own optimal working dilution for specific applications. |
| Applications | ELISA, Flow Cytometry, Immunofluorescence, Immunoprecipitation, Western Blot |
| Storage | This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at ï؟½80°C. Avoid Repeated Freeze Thaw Cycles. |
| Endotoxin | ≤ 1.0 EU/mg as determined by the LAL method |
| Purity | >95% by SDS-PAGE and HPLC |
| Formulation | This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (PBS) pH 7.2 - 7.4, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and azide free |
| Purification Method | This monoclonal antibody was purified using Protein A |
| Specificity | Clone 7G7B6 reacts with human CD25 |
| Species Reactivity | Human |
| Target | CD25 |
| Shipping Conditions | Blue ice |
| background | CD25 (IL-2R_) is the 55 kDa subunit of the interleukin 2 receptor alpha chain which has been shown to play roles in lymphocyte differentiation, activation, and proliferation. IL-2R_ is a transmembrane glycoprotein comprised of a heavily glycosylated extra |
| Other names | IL2RA, Interleukin-2 receptor subunit alpha, TAC, p55 |
| clone | 7G7B6 |
| UniProt | P01589 |
| Buffer | ICH3001-100ml ICH3002-100ml ICH3003-100ml |
| Antigen Distribution | IL-2R_ is expressed on activated mature T and B lymphocytes, during early stages of thymocytes development, pre-B cells, and in activated CD4+ memory T-lymphocytes |
| Immunogen | Recombinant human peripheral blood mononuclear cells (PBMCs) |
| Aggregation | Aggregation level ≤ 5% |
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