Anti-PD-1 (RMP1-14) 5mg/ml [ICH1132EC5]

PD-1

RMP1-14

Rat IgG2a

Programmed cell death protein 1, Pdcd1, CD279

Q02242

Rat

Mouse, Rat

Anti-PD-1 In Vivo Antibody – Low Endotoxin (RMP1-14) recognizes an epitope on Mouse PD-1

This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Subset of double negative thymocytes, activated T and B cells

CD279, also known as programmed death-1 (PD-1) is a 50-55 kD immunoglobulin superfamily member. PD-1 is expressed on a subset of CD4-CD8- thymocytes, and on activated T and B cells. The PD-1 ligands, PD-L1 (also known as B7-H1) and PD-L2 (B7-DC), are members of the B7 immunoglobulin superfamily. This RMP1-14 antibody has been reported to block the binding of PD-1 to its ligands (B7-H1 and B7-DC) and to inhibit T cell proliferation and cytokine production.

Mouse PD-1 transfected BHK cells

1.0 – 5.0 mg/ml

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and Azide free.

>95% by SDS-PAGE and HPLC
>98% by SDS-PAGE and HPLC

Endotoxin:

≤ 1.0 EU/mg as determined by the LAL method
≤ 0.75 EU/mg as determined by the LAL method

Aggregation:

Aggregation level ≤ 5%
Aggregation level ≤ 1%

This antibody is stable for at least 4 weeks when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 20°C or -80°C. Avoid repeated freeze thaw cycles.

Western Blot, Blocking, Flow Cytometry, Functional Assays

Blocking: We recommend using between 2.50 or 7.50 mg/kg when performing in vivo research using ichorbio’s PD-1 low endotoxin antibody clone RMP1-14. This range is based off the most recent publication data using the RMP1-14 clone in vivo.
Each investigator should determine their own optimal working dilution for specific applications.

Products are for research use only. Not for use in diagnostic or therapeutic procedures.