Anti-PD-1 (RMP1-14) 5mg/ml
Product Information
ichorbio’s anti-PD-1 (RMP1-14) 5mg/ml is sold at an exact concentration of 5mg/ml. This RMP1-14 in vivo antibody is available in the following sizes:
5mg, 25mg, 50mg and 100mg
ichorbio regularly manufactures bulk multi-gram amounts of our anti-PD-1 RMP1-14 clone – please contact us for pricing.
PD-1
RMP1-14
Rat IgG2a
Programmed cell death protein 1, Pdcd1, CD279
Rat
Mouse, Rat
Anti-PD-1 In Vivo Antibody – Low Endotoxin (RMP1-14) recognizes an epitope on Mouse PD-1
This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Subset of double negative thymocytes, activated T and B cells
CD279, also known as programmed death-1 (PD-1) is a 50-55 kD immunoglobulin superfamily member. PD-1 is expressed on a subset of CD4-CD8- thymocytes, and on activated T and B cells. The PD-1 ligands, PD-L1 (also known as B7-H1) and PD-L2 (B7-DC), are members of the B7 immunoglobulin superfamily. This RMP1-14 antibody has been reported to block the binding of PD-1 to its ligands (B7-H1 and B7-DC) and to inhibit T cell proliferation and cytokine production.
Mouse PD-1 transfected BHK cells
1.0 – 5.0 mg/ml
0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and Azide free.
>95% by SDS-PAGE and HPLC
>98% by SDS-PAGE and HPLC
Endotoxin:
≤ 1.0 EU/mg as determined by the LAL method
≤ 0.75 EU/mg as determined by the LAL method
Aggregation:
Aggregation level ≤ 5%
Aggregation level ≤ 1%
IMPACT Pathogen Test:
We use the IMPACT test generated by IDEXX Laboratories to guarantee our Ultra Low Endotoxin antibodies are pathogen free. Our rat antibodies are tested for:
Mycoplasma spp
Mycoplasma pulmonis
Pneumonia virus of mice
Kilham’s rat virus
Toolan’s H1 virus
Rat parvovirus
Lymphocytic choriomeningitis virus
Rat cytomegalovirus
Sendai virus
Rat coronavirus
Sialodacryoadenitis virus
Rat minute virus
Seoul virus
Mouse adenovirus
Reovirus 3
Rat theilovirus
This antibody is stable for at least 4 weeks when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 20°C or -80°C. Avoid repeated freeze thaw cycles.
Western Blot, Blocking, Flow Cytometry, Functional Assays
Blocking: We recommend using between 2.50 or 7.50 mg/kg when performing in vivo research using ichorbio’s PD-1 low endotoxin antibody clone RMP1-14. This range is based off the most recent publication data using the RMP1-14 clone in vivo.
Each investigator should determine their own optimal working dilution for specific applications.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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