Anti-Mouse MHC Class I H-2Kb (AF6-88.5) In Vivo Antibody - Low Endotoxin

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ICH1203
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$390.00

Anti-Mouse MHC Class I H-2Kb (AF6-88.5) In Vivo Antibody - Low Endotoxin

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Concentration ≤ 2.0 mg/ml
Isotype Mouse IgG2a _
Host Mouse
Use Products are for research use only. Not for use in diagnostic or therapeutic procedures.
Application Notes Each investigator should determine their own optimal working dilution for specific applications.
Applications Functional Assays, Flow Cytometry, IHC Frozen, Immunoprecipitation
Storage This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at ï؟½80°C. Avoid Repeated Freeze Thaw Cycles.
Endotoxin <1.0 EU/mg as determined by the LAL method
Purity >95% by SDS-PAGE
Formulation Sterile, preservative-free, solution in PBS. BSA and Azide free.
Purification Method This monoclonal antibody was purified using Protein A
Specificity Clone AF6-88.5, Anti-Mouse H-2Kb, recognizes an epitope on mouse MHC class I H-2K haplotype b. AF6-88.5 does not cross-react with other H-2K haplotypes, including d, f, j, k, p, q, r, s, u, v.
Species Reactivity Mouse
Target MHC Class I
Shipping Conditions Blue ice
clone AF6-88.5
Buffer ICH3001-100ml ICH3002-100ml ICH3003-100ml
Antigen Distribution The AF6-88.5 antibody reacts with H-2Kb MHC class I alloantigen and is ubiquitously expressed on nucleated cells from mice of the H-2Kb haplotype, including C57BL/6. H-2Kb is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins
Immunogen Mouse C57BL Splenocytes
Aggregation Aggregation level ≤ 5%
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