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Bulk Anti-Human CD16 (3G8) Antibody

Name: Bulk Anti-Human CD16 (3G8) Antibody
SKU: ICH1010
Price: 400 USD
Availability: InStock

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SKU

ICH1010

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$400.00 - $2,945.00

Bulk Anti-Human CD16 (3G8) Antibody

Endotoxin

≤ 1.0 EU/mg as determined by the LAL method

Purity

>95% by SDS-PAGE and HPLC

Species Reactivity

Human

Target

CD16

Concentration

≤ 2.0 mg/ml

Isotype

Mouse IgG1 Kappa

Host

Mouse

Use

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

Application Notes

Flow Cytometry: For flow cytometric staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume. Blocking: Clone 3G8 antibody blocks neutrophil phagocytosis and stimulates NK cell proliferation. Each investigator should determine their own optimal working dilution for specific applications.

Applications

IHC (Frozen), Flow Cytometry, Immunoprecipitation, Blocking, CyTOF

Storage

anti-CD16 In Vivo Antibody - Low Endotoxin (3G8) is stable when stored at 2-8°C.

Formulation

0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, without BSA or sodium azide added.

Purification Method

This monoclonal antibody was purified using Protein A

Specificity

Anti-CD16 In Vivo Antibody - Low Endotoxin (3G8) recognizes an epitope on Human CD16

Shipping Conditions

Blue ice

Background

CD16 (FcγRIII) is a low-affinity Fc receptor on NK cells, neutrophils and macrophages that triggers antibody-dependent cellular cytotoxicity. Clone 3G8 is a mouse IgG1 kappa monoclonal antibody against human CD16, validated for frozen-section IHC, flow cytometry, immunoprecipitation, blocking assays and CyTOF. Low endotoxin 3G8 is manufactured to in vivo standards and offered in scalable milligram (mg) to gram (g) quantities for high-throughput and heavy-usage research. Supplied strictly for research use only (RUO) as a non-therapeutic reagent, it provides dependable, lot-to-lot consistent performance for NK cell and Fc receptor biology.

Other Names

FCGR3A, Fc-gamma RIII, FcRIII

Clone

3G8

Uniprot

P08637

Buffer

ICH3001-100ml ICH3002-100ml ICH3003-100ml

Antigen Distribution

CD16 is expressed on the surface of NK cells, activated monocytes, macrophages, and placental trophoblasts in humans.

Immunogen

Human PMN cells