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Bulk Anti-Human CD16 (3G8) Antibody
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Bulk Anti-Human CD16 (3G8) Antibody
| Concentration | ≤ 2.0 mg/ml |
|---|---|
| Isotype | Mouse IgG1 Kappa |
| Host | Mouse |
| Use | Products are for research use only. Not for use in diagnostic or therapeutic procedures. |
| Application Notes | Flow Cytometry: For flow cytometric staining, the suggested use of this reagent is ï؟½2.0 µg per million cells in 100 ï؟½l volume. Blocking: Clone 3G8 antibody blocks neutrophil phagocytosis and stimulates NK cell proliferation. Each investigator should |
| Applications | IHC (Frozen), Flow Cytometry, Immunoprecipitation, Blocking, CyTOF |
| Storage | anti-CD16 In Vivo Antibody - Low Endotoxin (3G8) is stable when stored at 2-8°C. |
| Endotoxin | ≤ 1.0 EU/mg as determined by the LAL method |
| Purity | >95% by SDS-PAGE and HPLC |
| Formulation | 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, without BSA or sodium azide added. |
| Purification Method | This monoclonal antibody was purified using Protein A |
| Specificity | Anti-CD16 In Vivo Antibody - Low Endotoxin (3G8) recognizes an epitope on Human CD16 |
| Species Reactivity | Human |
| Target | CD16 |
| Shipping Conditions | Blue ice |
| background | CD16 is known as low affinity IgG receptor III (Fc gamma RIII) and is expressed as two distinct forms (CD16a and CD16b). CD16a (Fc gamma RIIIA) is a 50-65 kD polypeptide-anchored transmembrane protein. CD16b (Fc gamma RIIIB) is a 48 kD glycosylphosphatidy |
| Other names | FCGR3A, Fc-gamma RIII, FcRIII |
| clone | 3G8 |
| UniProt | P08637 |
| Buffer | ICH3001-100ml ICH3002-100ml ICH3003-100ml |
| Antigen Distribution | CD16 is expressed on the surface of NK cells, activated monocytes, macrophages, and placental trophoblasts in humans. |
| Immunogen | Human PMN cells |
| Aggregation | Aggregation level ≤ 5% |
| Antibodies against the same target | Anti-CD32/CD16 In Vivo Antibody - Low Endotoxin [2.4G2] (ICH1056) |
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