Human CD32b Protein (In Vivo Grade, Ultra-Low Endotoxin)

Human

Human CD32b Protein

Human embryonic kidney (HEK) 293 cells.

P31994

Ambient

CD32b (FcγRIIb) is the principal inhibitory Fc gamma receptor, a key brake on B-cell and myeloid-cell activation that shapes antibody effector function and immune-complex clearance. This recombinant human CD32b ectodomain is expressed in human embryonic kidney (HEK) 293 cells and validated for SPR, BLI and ELISA, making it well suited to mapping antibody Fc binding, profiling Fc engineering variants and characterizing immune-complex interactions. Supplied as a research-use-only (RUO) reagent in an ultra-low-endotoxin, in vivo grade format, it is produced in scalable milligram (mg) to gram (g) quantities to support bulk binding studies and high-throughput Fc receptor screening with reliable lot-to-lot consistency.

Human Fc gamma RIIb, CD32B, FCGR2B, FCGRIIB, FCR2B, FCRIIB, IGFR2B, IGFRIIB

The sequence of the extracellular domain of human CD32b (Ala 46-Pro 217) was fused with a C-terminal tag consisting of the AVI tag, TEV protease recognition sequence and a 10-His tag.

Lyophilized from sterile PBS, pH 7.4. No preservatives or cryoprotectants have been added. To obtain a final concentration of 1 mg/ml reconstitute 0.25 mg vials with 0.25 ml water and 1.0 mg vials with 1.0 ml water.

Lyophilized proteins are stable at ambient temperature for at least 2 weeks. If the protein is not to be used immediately then the protein should be stored in lyophilized form at -20 °C for up 12 months. Once the protein has been reconstituted we recommen

SPR, BLI, ELISA

Products are for research use only. Not for use in diagnostic or therapeutic procedures.