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Human CD32a (167R) Protein (In Vivo Grade, Ultra-Low Endotoxin)
Human CD32a (167R) Protein (In Vivo Grade, Ultra-Low Endotoxin)
Endotoxin
<1.0 EU per mg as determined by the LAL method.
Purity
>95% monomer purity as determined by SDS-PAGE and SEC-HPLC.
Species Reactivity
Human
Target
Human CD32a (167R) Protein
Host
Human embryonic kidney (HEK) 293 cells.
Uniprot
P12318
Shipping Conditions
Ambient
Background
Among Fc-gamma receptors, the 167R (arginine) variant of CD32a (FcgammaRIIa) differs from the 167H allele in immune-complex binding behavior, making allelic comparison a recurring theme in Fc-receptor research. This product is a recombinant human CD32a (167R) protein, not an antibody, expressed in HEK293 cells and validated for SPR, BLI, and ELISA studies. Manufactured to in vivo-grade, ultra-low-endotoxin standards, it is supplied in scalable milligram to gram quantities for bulk and high-throughput laboratories. Researchers employ the 167R allelic variant as a defined binding partner for Fc-receptor interaction assays, polymorphism comparisons, kinetic characterization, and assay development, with the protein provided strictly for research use only.
Other Names
Human Fc gamma RIIa, CD32A, FCGR2A, FCGRIIA, FCR2A, FCRIIA, IGFR2A, IGFRIIA
Specificity
The sequence of the extracellular domain of human CD32a (Ala 36-Ile 218) was fused with a C-terminal tag consisting of the AVI tag, TEV protease recognition sequence and a 10-His tag.
Formulation
Lyophilized from sterile PBS, pH 7.4. No preservatives or cryoprotectants have been added. To obtain a final concentration of 1 mg/ml reconstitute 0.25 mg vials with 0.25 ml water and 1.0 mg vials with 1.0 ml water.
Storage
Lyophilized proteins are stable at ambient temperature for at least 2 weeks. If the protein is not to be used immediately then the protein should be stored in lyophilized form at -20 °C for up 12 months. Once the protein has been reconstituted we recommen
Applications
SPR, BLI, ELISA
Use
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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