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Bulk Anti-Human CD14 (UCHM-1) Antibody
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Bulk Anti-Human CD14 (UCHM-1) Antibody
| Concentration | 1.0 - 5.0 mg/ml |
|---|---|
| Isotype | Mouse IgG2a |
| Host | Mouse |
| Use | Products are for research use only. Not for use in diagnostic or therapeutic procedures. |
| Application Notes | Each investigator should determine their own optimal working dilution for specific applications. |
| Applications | Flow Cytometry, Western Blot, IHC (Frozen), Immunofluorescence, Immunoprecipitation |
| Storage | anti-CD14 In Vivo Antibody - Low Endotoxin (UCHM-1) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at ï؟½80°C. Avoid Repeated Freeze Thaw Cycles. |
| Endotoxin | ≤ 1.0 EU/mg as determined by the LAL method |
| Purity | >95% by SDS-PAGE and analyzed by silver stain. |
| Formulation | 0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. |
| Purification Method | This monoclonal antibody was purified using Protein A |
| Specificity | Anti-CD14 In Vivo Antibody - Low Endotoxin (UCHM-1) recognizes Human CD14. Anti-Human CD14 recognizes a (Mr 55 kDa) cell surface glycoprotein expressed on most peripheral blood monocytes. |
| Species Reactivity | Human |
| Target | CD14 |
| Shipping Conditions | Blue ice |
| background | Cluster of differentiation 14 also known as CD14 is a cell surface glycoprotein expressed in monocytes, macrophages, polymorphonuclear neutrophils, chondrocytes, B-cells, dendritic cells. CD14 functions to enhance activation of cells by lipopolysaccharide |
| Other names | Myeloid cell-specific leucine-rich glycoprotein |
| clone | UCHM-1 |
| UniProt | P08571 |
| Buffer | ICH3001-100ml ICH3002-100ml ICH3003-100ml |
| Antigen Distribution | The CD14 antigen is expressed on approximately 70-95% of human peripheral blood monocytes. It is also found on other cells of myeloid lineage including granulocytes, dendritic reticulum cells and pleural phagocytic cells. |
| Aggregation | Aggregation level ≤ 5% |
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