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Bulk Anti-Human CD14 (UCHM-1) Antibody

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SKU

ICH1009

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$390.00

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Bulk anti-Human CD14 antibody (UCHM-1)

Product Benefits:

ichorbio's bulk anti-human CD14 antibody (UCHM-1) is manufactured in a cGMP compliant, ISO Quality Standard 9001:2015 facility. ichorbio's low endotoxin antibodies have half the endotoxin of comparable antibodies from Bio X Cell at less than 1.0 EU/mg. If ichorbio's low endotoxin antibodies are not low enough we also offer ultra low endotoxin antibodies which have even less endotoxin (<0.5EU/mg) at an even higher purity (98% versus 95%). ichorbio: the best antibodies for in vivo research.

Target:

CD14

Clone:

UCHM-1

Isotype:

Mouse IgG2a

Other Names:

Myeloid cell-specific leucine-rich glycoprotein

Uniprot:

P08571

Host:

Mouse

Species Reactivity:

Human

Specificity:

Bulk anti-human CD14 antibody (UCHM-1) recognizes Human CD14. Anti-Human CD14 recognizes a (Mr 55 kDa) cell surface glycoprotein expressed on most peripheral blood monocytes.

Purification Method:

This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Antigen Distribution:

The CD14 antigen is expressed on approximately 70-95% of human peripheral blood monocytes. It is also found on other cells of myeloid lineage including granulocytes, dendritic reticulum cells and pleural phagocytic cells.

Background:

Cluster of differentiation 14 also known as CD14 is a cell surface glycoprotein expressed in monocytes, macrophages, polymorphonuclear neutrophils, chondrocytes, B-cells, dendritic cells. CD14 functions to enhance activation of cells by lipopolysaccharide (LPS).

Concentration:

1.0 - 5.0 mg/ml

Formulation:

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and Azide free.

Purity:

>95% by SDS-PAGE and analyzed by silver stain.

>98% by SDS-PAGE and HPLC

Endotoxin:

≤ 1.0 EU/mg as determined by the LAL method

≤ 0.75 EU/mg as determined by the LAL method

Aggregation:

Aggregation level ≤ 5%

Aggregation level ≤ 1%

Storage:

This antibody is stable for at least 4 weeks when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 20°C or -80°C. Avoid repeated freeze thaw cycles.

Applications:

Flow Cytometry, Western Blot, IHC (Frozen), Immunofluorescence, Immunoprecipitation

Application Notes:

Each investigator should determine their own optimal working dilution for specific applications.

Use:

Products are for research use only.

Isotype Control:

Mouse IgG2a In Vivo Isotype Control - Low Endotoxin [C1.18.4] (ICH2245)

More Information
Concentration	1.0 - 5.0 mg/ml
Isotype	Mouse IgG2a
Host	Mouse
Use	Products are for research use only. Not for use in diagnostic or therapeutic procedures.
Application Notes	Each investigator should determine their own optimal working dilution for specific applications.
Applications	Flow Cytometry, Western Blot, IHC (Frozen), Immunofluorescence, Immunoprecipitation
Storage	anti-CD14 In Vivo Antibody - Low Endotoxin (UCHM-1) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at ï؟½80°C. Avoid Repeated Freeze Thaw Cycles.
Endotoxin	≤ 1.0 EU/mg as determined by the LAL method
Purity	>95% by SDS-PAGE and analyzed by silver stain.
Formulation	0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.
Purification Method	This monoclonal antibody was purified using Protein A
Specificity	Anti-CD14 In Vivo Antibody - Low Endotoxin (UCHM-1) recognizes Human CD14. Anti-Human CD14 recognizes a (Mr 55 kDa) cell surface glycoprotein expressed on most peripheral blood monocytes.
Species Reactivity	Human
Target	CD14
Shipping Conditions	Blue ice
background	Cluster of differentiation 14 also known as CD14 is a cell surface glycoprotein expressed in monocytes, macrophages, polymorphonuclear neutrophils, chondrocytes, B-cells, dendritic cells. CD14 functions to enhance activation of cells by lipopolysaccharide
Other names	Myeloid cell-specific leucine-rich glycoprotein
clone	UCHM-1
UniProt	P08571
Buffer	ICH3001-100ml ICH3002-100ml ICH3003-100ml
Antigen Distribution	The CD14 antigen is expressed on approximately 70-95% of human peripheral blood monocytes. It is also found on other cells of myeloid lineage including granulocytes, dendritic reticulum cells and pleural phagocytic cells.
Aggregation	Aggregation level ≤ 5%