Bulk Anti-Human CD14 (UCHM-1) Antibody
Bulk anti-Human CD14 antibody (UCHM-1)
Product Benefits:ichorbio's bulk anti-human CD14 antibody (UCHM-1) is manufactured in a cGMP compliant, ISO Quality Standard 9001:2015 facility. ichorbio's low endotoxin antibodies have half the endotoxin of comparable antibodies from Bio X Cell at less than 1.0 EU/mg. If ichorbio's low endotoxin antibodies are not low enough we also offer ultra low endotoxin antibodies which have even less endotoxin (<0.5EU/mg) at an even higher purity (98% versus 95%). ichorbio: the best antibodies for in vivo research.
Target:CD14
Clone:UCHM-1
Isotype:Mouse IgG2a
Other Names:Myeloid cell-specific leucine-rich glycoprotein
Uniprot: Host:Mouse
Species Reactivity:Human
Specificity:Bulk anti-human CD14 antibody (UCHM-1) recognizes Human CD14. Anti-Human CD14 recognizes a (Mr 55 kDa) cell surface glycoprotein expressed on most peripheral blood monocytes.
Purification Method:This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Antigen Distribution:The CD14 antigen is expressed on approximately 70-95% of human peripheral blood monocytes. It is also found on other cells of myeloid lineage including granulocytes, dendritic reticulum cells and pleural phagocytic cells.
Background:Cluster of differentiation 14 also known as CD14 is a cell surface glycoprotein expressed in monocytes, macrophages, polymorphonuclear neutrophils, chondrocytes, B-cells, dendritic cells. CD14 functions to enhance activation of cells by lipopolysaccharide (LPS).
Concentration:1.0 - 5.0 mg/ml
Formulation:0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and Azide free.
Purity:>95% by SDS-PAGE and analyzed by silver stain.
>98% by SDS-PAGE and HPLC
Endotoxin:≤ 1.0 EU/mg as determined by the LAL method
≤ 0.75 EU/mg as determined by the LAL method
Aggregation:Aggregation level ≤ 5%
Aggregation level ≤ 1%
Storage:This antibody is stable for at least 4 weeks when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 20°C or -80°C. Avoid repeated freeze thaw cycles.
Applications:Flow Cytometry, Western Blot, IHC (Frozen), Immunofluorescence, Immunoprecipitation
Application Notes:Each investigator should determine their own optimal working dilution for specific applications.
Use:Products are for research use only.
Isotype Control:Mouse IgG2a In Vivo Isotype Control - Low Endotoxin [C1.18.4] (ICH2245)
Concentration | 13.3mg/ml |
---|---|
isotype | Mouse IgG2a |
Host | Mouse |