Bulk Human IgG1 (IB1) Isotype Control D265A/N297A

Human

IgG1

≥ 5.0 mg/ml

Human IgG1

CHO

Aggregation level ≤ 5%

ICH3001-100ml ICH3002-100ml ICH3003-100ml

P01857

Blue ice

The D265A/N297A variant of clone IB1 is a human IgG1 isotype control carrying paired Fc mutations that abolish FcgammaR and complement engagement, making it an effector-silent, non-binding reference for human IgG1 experiments. It supports flow cytometry and functional assays as a background, gating, and negative-control reagent. The antibody is supplied in a low-endotoxin format suitable for in vivo use and manufactured in scalable milligram to gram quantities for bulk and high-throughput laboratories. It is non-therapeutic and provided for research use only, ensuring reproducible, effector-null control performance.

IB1

This Human IgG1 isotype control recombinant antibody has been tested against selected species' cells and tissues to assure minimal cross reactivity. Furthermore, this low endotoxin Human IgG1 antibody is suitable for in vivo applications and each lot is IMPACT I certified.

This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.

This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

Flow Cytometry, Functional Assays

Each investigator should determine their own optimal working dilution for specific applications.

Products are for research use only. Not for use in diagnostic or therapeutic procedures.