Bulk Anti-DYKDDDDK Tag (M2-ICH) Antibody

FLAG tag, M2 clone, DYKDDDDK peptide

ICH3001-100ml ICH3002-100ml ICH3003-100ml

DYKDDDDK-tag

Mouse IgG1

Mouse

1mg/ml

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

ELISA, flow cytometry, immunoblotting, immunoprecipitation, immunocytochemistry, immunofluorescence, chromatin immunoprecipitation, electron microscopy, supershift assays, protein purification if conjugated with agarose or magnetic beads, and isotype cont

This low endotoxin FLAG-tag antibody is stable when stored at 2-8°C for at least four (4) weeks. For long-term storage aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

0.01 M phosphate buffered saline (PBS) pH 7.2 - 7.4, 150 mM NaCl with no carrier protein, potassium or preservatives added.

This monoclonal antibody was purified using Protein A

Alternative of the monoclonal FLAG M2 antibody for the detection and purification of DYKDDDDK fusion proteins no matter if the DYKDDDDK sequence is present at the N-terminus, Met N-terminus, C-terminus, or an internal site of the fusion proteins. FLAG is a registered trade mark of Sigma Aldrich, and used here for informational purposes only.

Blue ice

The DYKDDDDK (FLAG) tag is a short hydrophilic peptide widely fused to recombinant proteins for detection, immunoprecipitation, and purification. This mouse IgG1 antibody is a recombinant version of the M2 clone, recognising the DYKDDDDK-tag, with broad utility across ELISA, immunoblotting, immunoprecipitation, flow cytometry, immunofluorescence, ChIP, and tagged-protein purification. The M2-ICH anti-FLAG reagent is supplied in a low-endotoxin format suitable for in vivo use and manufactured in scalable milligram to gram quantities for bulk and high-throughput laboratories. It is intended for research use only as a non-therapeutic reagent and delivers reliable, reproducible tag detection lot after lot.

Recombinant version of the M2 clone

DYKDDDDK tag