Anti-Mouse Dendritic Cells (33D1) In Vivo Antibody - Low Endotoxin
Bulk anti-Dendritic Cells In Vivo Antibody - Low Endotoxin (33D1)
Product Benefits:ichorbio's anti-Dendritic Cells In Vivo Antibody - Low Endotoxin (33D1) is manufactured in a cGMP compliant, ISO Quality Standard 9001:2015 facility. ichorbio's low endotoxin antibodies have half the endotoxin of comparable antibodies from Bio X Cell at less than 1.0 EU/mg. If ichorbio's low endotoxin antibodies are not low enough we also offer ultra low endotoxin antibodies which have even less endotoxin (<0.5EU/mg) at an even higher purity (98% versus 95%). ichorbio: the best antibodies for in vivo research.
Target:Dendritic Cells
Clone:33D1
Isotype:Rat IgG2b
Other Names:DCIR2
Host:Rat
Species Reactivity:Mouse
Specificity:Anti-Dendritic Cells In Vivo Antibody - Low Endotoxin (33D1) recognizes Mouse Dendritic Cells
Purification Method:This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Antigen Distribution:Clone 33D1 monoclonal antibody reacts with mouse dendritic cell (DC), specific surface marker, and DCIR2. DCIR2 has been reported on a variety of dendritic cell subpopulations from mouse thymus, spleen, lymph node, and Peyer's patch. Bone marrow dendritic cells require GM-CSF to express DCIR2, and this expression is downregulated in the presence of IL-4. DCIR2 has been detected in vivo in brain dendritic cells post infection with Toxoplasma gondii.
Background:Dendritic cells (DC) are a small subpopulation of lymphoid cells with distinctive cytologic features, surface properties, and functions.
Immunogen:Dendritic cells
Concentration:0.5 mg/ml
Formulation:0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and Azide free.
Purity:>95% by SDS-PAGE and HPLC
>98% by SDS-PAGE and HPLC
Endotoxin:≤ 1.0 EU/mg as determined by the LAL method
≤ 0.75 EU/mg as determined by the LAL method
Aggregation:Aggregation level ≤ 5%
Aggregation level ≤ 1%
Storage:This antibody is stable for at least 4 weeks when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 20°C or -80°C. Avoid repeated freeze thaw cycles.
Applications:Western Blot, IHC (Frozen), Flow Cytometry, Immunocytochemistry
Application Notes:Each investigator should determine their own optimal working dilution for specific applications.
Use:Products are for research use only.
Isotype Control:Rat IgG2b In Vivo Isotype Control - Low Endotoxin [1-2] (ICH2243)
| Concentration | 0.5 mg/ml |
|---|---|
| Isotype | Rat IgG2b |
| Host | Rat |
| Use | Products are for research use only. Not for use in diagnostic or therapeutic procedures. |
| Application Notes | Each investigator should determine their own optimal working dilution for specific applications. |
| Applications | Western Blot, IHC (Frozen), Flow Cytometry, Immunocytochemistry |
| Storage | anti-Dendritic Cells In Vivo Antibody - Low Endotoxin (33D1) is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at ≤ 20°C in a manual defrost freezer. Avoid Repeated Fre |
| Endotoxin | ≤ 1.0 EU/mg as determined by the LAL method |
| Purity | >95% by SDS-PAGE and HPLC |
| Formulation | No carrier proteins or preservatives added in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl. |
| Purification Method | This monoclonal antibody was purified using Protein G |
| Specificity | Anti-Dendritic Cells In Vivo Antibody - Low Endotoxin (33D1) recognizes Mouse Dendritic Cells |
| Species Reactivity | Mouse |
| Target | Dendritic Cells |
| Shipping Conditions | Blue ice |
| background | Dendritic cells (DC) are a small subpopulation of lymphoid cells with distinctive cytologic features, surface properties, and functions. |
| Other names | DCIR2 |
| clone | 33D1 |
| Buffer | ICH3001-100ml ICH3002-100ml ICH3003-100ml |
| Antigen Distribution | Clone 33D1 monoclonal antibody reacts with mouse dendritic cell (DC), specific surface marker, and DCIR2. DCIR2 has been reported on a variety of dendritic cell subpopulations from mouse thymus, spleen, lymph node, and Peyer's patch. Bone marrow dendritic |
| Immunogen | Dendritic cells |
| Aggregation | Aggregation level ≤ 5% |