Anti-Mouse Dendritic Cells (33D1) In Vivo Antibody - Low Endotoxin

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ICH1092
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$390.00

Bulk anti-Dendritic Cells In Vivo Antibody - Low Endotoxin (33D1)

  Product Benefits:

ichorbio's anti-Dendritic Cells In Vivo Antibody - Low Endotoxin (33D1) is manufactured in a cGMP compliant, ISO Quality Standard 9001:2015 facility. ichorbio's low endotoxin antibodies have half the endotoxin of comparable antibodies from Bio X Cell at less than 1.0 EU/mg. If ichorbio's low endotoxin antibodies are not low enough we also offer ultra low endotoxin antibodies which have even less endotoxin (<0.5EU/mg) at an even higher purity (98% versus 95%). ichorbio: the best antibodies for in vivo research.

Target:

Dendritic Cells

Clone:

33D1

Isotype:

Rat IgG2b

Other Names:

DCIR2

Host:

Rat

Species Reactivity:

Mouse

Specificity:

Anti-Dendritic Cells In Vivo Antibody - Low Endotoxin (33D1) recognizes Mouse Dendritic Cells

Purification Method:

This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Antigen Distribution:

Clone 33D1 monoclonal antibody reacts with mouse dendritic cell (DC), specific surface marker, and DCIR2. DCIR2 has been reported on a variety of dendritic cell subpopulations from mouse thymus, spleen, lymph node, and Peyer's patch. Bone marrow dendritic cells require GM-CSF to express DCIR2, and this expression is downregulated in the presence of IL-4. DCIR2 has been detected in vivo in brain dendritic cells post infection with Toxoplasma gondii.

Background:

Dendritic cells (DC) are a small subpopulation of lymphoid cells with distinctive cytologic features, surface properties, and functions.

Immunogen:

Dendritic cells

Concentration:

0.5 mg/ml

Formulation:

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and Azide free.

Purity:

>95% by SDS-PAGE and HPLC

>98% by SDS-PAGE and HPLC

Endotoxin:

≤ 1.0 EU/mg as determined by the LAL method

≤ 0.75 EU/mg as determined by the LAL method

Aggregation:

Aggregation level ≤ 5%

Aggregation level ≤ 1%

Storage:

This antibody is stable for at least 4 weeks when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 20°C or -80°C. Avoid repeated freeze thaw cycles.

Applications:

Western Blot, IHC (Frozen), Flow Cytometry, Immunocytochemistry

Application Notes:

Each investigator should determine their own optimal working dilution for specific applications.

Use:

Products are for research use only.

Isotype Control:

Rat IgG2b In Vivo Isotype Control - Low Endotoxin [1-2] (ICH2243)

More Information
Concentration 0.5 mg/ml
Isotype Rat IgG2b
Host Rat
Use Products are for research use only. Not for use in diagnostic or therapeutic procedures.
Application Notes Each investigator should determine their own optimal working dilution for specific applications.
Applications Western Blot, IHC (Frozen), Flow Cytometry, Immunocytochemistry
Storage anti-Dendritic Cells In Vivo Antibody - Low Endotoxin (33D1) is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at ≤ 20°C in a manual defrost freezer. Avoid Repeated Fre
Endotoxin ≤ 1.0 EU/mg as determined by the LAL method
Purity >95% by SDS-PAGE and HPLC
Formulation No carrier proteins or preservatives added in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl.
Purification Method This monoclonal antibody was purified using Protein G
Specificity Anti-Dendritic Cells In Vivo Antibody - Low Endotoxin (33D1) recognizes Mouse Dendritic Cells
Species Reactivity Mouse
Target Dendritic Cells
Shipping Conditions Blue ice
background Dendritic cells (DC) are a small subpopulation of lymphoid cells with distinctive cytologic features, surface properties, and functions.
Other names DCIR2
clone 33D1
Buffer ICH3001-100ml ICH3002-100ml ICH3003-100ml
Antigen Distribution Clone 33D1 monoclonal antibody reacts with mouse dendritic cell (DC), specific surface marker, and DCIR2. DCIR2 has been reported on a variety of dendritic cell subpopulations from mouse thymus, spleen, lymph node, and Peyer's patch. Bone marrow dendritic
Immunogen Dendritic cells
Aggregation Aggregation level ≤ 5%
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