Anti-Mouse CD90.1 (OX-7) In Vivo Antibody - Low Endotoxin

Mouse, Rat

CD90.1

>1mg/ml

Mouse IgG1

Mouse

Aggregation level ≤ 5%

CD90 is present on a variety of cell types in murine and rat, including lymphatic vessels, T cells, neurons and fibroblasts

ICH3001-100ml ICH3002-100ml ICH3003-100ml

Blue ice

Clone OX-7 is a mouse IgG1 monoclonal antibody that recognises the allelic marker CD90.1 (Thy-1.1) and cross-reacts with rat CD90, while showing no reactivity to the CD90.2 (Thy-1.2) allele carried by most common inbred strains. This allotype specificity is what makes OX-7 particularly useful for in-vivo work: in adoptive-transfer and bone-marrow chimera designs, CD90.1-marked donor cells can be tracked, gated, or selectively depleted in a CD90.2 host without touching endogenous compartments. This ichor.bio preparation is supplied low-endotoxin (research grade <1 EU/mg; ultra-low <0.5 EU/mg), purified, and offered in bulk milligram-to-gram quantities suited to repeat-dosing animal studies. It is validated for depletion alongside flow cytometry, immunofluorescence, and immunohistochemistry, so the same clone can be used to deplete and then confirm depletion. The format supports functional in-vivo cell-ablation and cell-tracking experiments as well as ex-vivo phenotyping. For research use only (RUO); not for diagnostic or therapeutic use. All content here is guidance for ichorbio to review before publishing. CD90.1 (Thy-1.1) is one of two allelic forms of Thy-1, a small glycosylphosphatidylinositol (GPI)-anchored cell-surface glycoprotein of the immunoglobulin superfamily. Thy-1 is expressed broadly on mouse thymocytes and peripheral T cells, on subsets of haematopoietic stem and progenitor cells, and on neurons, fibroblasts, and other stromal populations. The CD90.1 versus CD90.2 distinction is an allotypic polymorphism: CD90.1 is carried by strains such as AKR/J, PL, and FVB/N, whereas most laboratory strains (e.g. C57BL/6, BALB/c) carry CD90.2. Because the two alleles are congenically distinguishable, CD90.1 is widely exploited as a lineage-tracing and congenic marker to separate transferred donor cells from host cells. Functionally, Thy-1 participates in T-cell activation, cell-cell and cell-matrix adhesion, and neurite outgrowth, though its role as an allelic marker dominates its experimental use.

OX 7, OX7

OX-7

Clone OX-7 activity is directed against both rat and murine CD90/Thy-1.

This monoclonal antibody was purified using Protein A

Sterile, preservative-free, solution in PBS. BSA and Azide free.

This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

Depletion, Flow Cytometry, Immunofluorescence, Immunohistochemistry

Each investigator should determine their own optimal working dilution for specific applications.

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

Q: Is clone OX-7 suitable for in vivo CD90.1 studies?

A: Yes - OX-7 is a low-endotoxin, in vivo-grade antibody suitable for in vivo CD90.1 studies and in vitro work.

Q: Can I order anti-mouse CD90.1 clone OX-7 in bulk?

A: Yes, it is manufactured in scalable milligram to gram quantities for bulk and high-throughput experiments.

Q: What does clone OX-7 target?

A: It is an anti-CD90.1 (Thy-1.1) monoclonal used to identify and study cells expressing the Thy-1.1 allele; for research use only.