Anti-ICAM-1 (15.2) In Vivo Antibody - Low Endotoxin

In stock

Only %1 left

SKU

ICH1027

Add to Compare

Rating:

100 % of 100

1 Review Add Your Review

$380.00

Skip to the end of the images gallery

Skip to the beginning of the images gallery

Add to Wish List

Bulk anti-ICAM-1 antibody clone 15.2

Product Benefits:

ichorbio's anti-ICAM-1 In Vivo Antibody - Low Endotoxin (15.2) [ICH1027] is manufactured in a cGMP compliant, ISO Quality Standard 9001:2015 facility. ichorbio's low endotoxin antibodies have half the endotoxin of comparable antibodies from Bio X Cell at less than 1.0 EU/mg. If ichorbio's low endotoxin antibodies are not low enough we also offer ultra low endotoxin antibodies which have even less endotoxin (<0.5EU/mg) at an even higher purity (98% versus 95%). ichorbio: the best antibodies for in vivo research.

Target:

ICAM-1

Clone:

15.2

Isotype:

Mouse IgG1

Other Names:

Intercellular adhesion molecule 1, ICAM1, Major group rhinovirus receptor, CD54

Host:

Mouse

Species Reactivity:

Human, Mouse, Rat

Specificity:

Mouse anti-ICAM-1 in vivo antibody - low endotoxin (15.2) recognizes an epitope on Human ICAM-1

Purification Method:

This bulk anti-ICAM-1 antibody clone 15.2 was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Concentration:

0.5 mg/ml

Formulation:

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and Azide free.

Purity:

>95% by SDS-PAGE and HPLC

>98% by SDS-PAGE and HPLC

Endotoxin:

≤ 1.0 EU/mg as determined by the LAL method

≤ 0.75 EU/mg as determined by the LAL method

Aggregation:

Aggregation level ≤ 5%

Aggregation level ≤ 1%

Storage:

This antibody is stable for at least 4 weeks when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 20°C or -80°C. Avoid repeated freeze thaw cycles.

Applications:

IHC (FFPE), Flow Cytometry, Immunoprecipitation, Western Blot

Application Notes:

Each investigator should determine their own optimal working dilution for specific applications.

Use:

Products are for research use only.

Antibodies against the same target:

Anti-ICAM-1 In Vivo Antibody - Low Endotoxin [BE29G1] (ICH1101)

Immunofluorescence (paraffin-embedded sections):

Immunofluorescence analysis of paraffin-embedded breast cancer tissue section labeling ICAM-1 (1:100) overnight at 4˚, followed by goat anti-mouse IgG H&L (Alexa Fluor ® 647-red) secondary antibody (1:500 dilution). MDA-MB-231 cells were injected via mouse nipple and grown for 5 weeks. The nuclear counter stain is DAPI (blue). Image was acquired on a Nikon A1R microscope system at 4x magnification (first image) or 60x magnification (second image).

Alternative Names: