Anti-CTLA-4 In Vivo Antibody, Low Endotoxin, UC10-4F10-11

Bulk anti-CTLA-4 In Vivo Antibody - Low Endotoxin (UC10-4F10-11)

Bio X Cell:

ICH1085 is up to 37% cheaper for industry than the equivalent product BE0032 from Bio X Cell. Product Benefits:

ichorbio's anti-CTLA-4 In Vivo Antibody - Low Endotoxin (UC10-4F10-11) is manufactured in a cGMP compliant, ISO Quality Standard 9001:2015 facility. ichorbio's low endotoxin antibodies have half the endotoxin of comparable antibodies from Bio X Cell at less than 1.0 EU/mg. If ichorbio's low endotoxin antibodies are not low enough we also offer ultra low endotoxin antibodies which have even less endotoxin (<0.5EU/mg) at an even higher purity (98% versus 95%). ichorbio: the best antibodies for in vivo research.

Target:

CTLA-4

Clone:

UC10-4F10-11

Size:

ichorbio's UC10-4F10-11 in vivo antibody is available in the following bulk sizes: 1mg, 5mg, 25mg, 50mg and 100mg ichorbio regularly manufactures multi-gram amounts of our anti-CTLA-4 UC10-4F10-11 clone - please contact us for pricing.

Isotype:

Armenian Hamster IgG1

Other Names:

Ctla4, Cytotoxic T-lymphocyte protein 4, CD152

Uniprot:

P09793

Host:

Armenian Hamster

Species Reactivity:

Mouse

Specificity:

Anti-CTLA-4 In Vivo Antibody - Low Endotoxin (UC10-4F10-11) recognizes an epitope on Mouse CD152

Purification Method:

This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Antigen Distribution:

The UC10-4F10-11 antibody reacts with CD152 (CTLA-4), which is expressed on activated T lymphocytes 2-3 days after stimulation through T cell receptor.

Background:

CD152, also known as CTLA-4 or Ly-56 has significant similarity to CD28 in amino acid sequence, structure, and genomic organization. Furthermore, CD152 and CD28 share common B7 family counter-receptors. Unlike CD28, CD152 expression appears to be restricted to activated T cells and CD25+CD4+ regulatory T (Treg) cells. CD152 is thought to play a role in the induction and maintenance of immunological tolerance as well as the development of protective immunity and thymocyte regulation.

Immunogen:

Purified Recombinant Mouse CTLA4 Fc Fusion Protein

Concentration:

≥ 2.0 mg/ml

Formulation:

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and Azide free.

Purity:

>95% by SDS-PAGE and HPLC

>98% by SDS-PAGE and HPLC

Endotoxin:

≤ 1.0 EU/mg as determined by the LAL method

≤ 0.75 EU/mg as determined by the LAL method

Aggregation:

Aggregation level ≤ 5%

Aggregation level ≤ 1%

Storage:

This antibody is stable for at least 4 weeks when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 20°C or -80°C. Avoid repeated freeze thaw cycles.

Applications:

Flow Cytometry, Western Blot, Functional Assays, Immunoprecipitation

in vivo

We recommend using between 50-250µg per mouse when performing in vivo research using ichorbio’s CTLA-4 low endotoxin antibody clone UC10-4F10-11. This range is based off the most recent publication data using the UC10-4F10-11 clone in vivo.

Application Notes

Functional Assay: Clone UC10-4F10-11 antibody has been shown to promote T cell co-stimulation by blocking CTLA-4 binding to the B7 co-receptors, allowing for CD28 binding. Each investigator should determine their own optimal working dilution for specific applications.

Use:

Products are for research use only.

Isotype Control:

Armenian Hamster IgG Isotype Control for In Vivo - Low Endotoxin [PIP] (ICH2251)

Antibodies against the same target:

Anti-CTLA-4 In Vivo Antibody - Low Endotoxin [9H10] (ICH1084), Anti-CTLA-4 In Vivo Antibody - Ultra Low Endotoxin [9D9] (ICH1096UL), Anti-CTLA-4 In Vivo Antibody - Low Endotoxin [9D9] (ICH1096), Anti-CTLA-4 In Vivo Antibody - Ultra Low Endotoxin [9H10] (ICH1084UL)

Alternative Names:

ALPS5 antibody
CD 152 antibody
CD antibody
CD152 antibody
CD152 antigen antibody
CD152 isoform antibody
Celiac disease 3 antibody
CELIAC3 antibody
CTLA 4 antibody
CTLA-4 antibody
CTLA4 antibody
Cytotoxic T cell associated 4 antibody
Cytotoxic T lymphocyte antigen 4 antibody
Cytotoxic T lymphocyte associated 4 antibody
Cytotoxic T lymphocyte associated 4, soluble isoform, included antibody
Cytotoxic T lymphocyte associated antigen 4 antibody
Cytotoxic T lymphocyte associated antigen 4 short spliced form antibody
Cytotoxic T lymphocyte associated protein 4 antibody
Cytotoxic T lymphocyte associated serine esterase 4 antibody
Cytotoxic T lymphocyte protein 4 antibody
Cytotoxic T-lymphocyte protein 4 antibody
Cytotoxic T-lymphocyte-associated antigen 4 antibody
GRD4 antibody
GSE antibody
ICOS antibody
IDDM12 antibody
Insulin-dependent diabetes mellitus 12 antibody
Ligand and transmembrane spliced cytotoxic T lymphocyte associated antigen 4 antibody
OTTHUMP00000216623 antibody