Pig CD32a Protein (In Vivo Grade, Ultra-Low Endotoxin)

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SKU
ICH3021
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$1,500.00

Pig CD32a Protein (In Vivo Grade, Ultra-Low Endotoxin)

Endotoxin
<1.0 EU per mg as determined by the LAL method.
Purity
>95% monomer purity as determined by SDS-PAGE and SEC-HPLC.
Species Reactivity
Pig (sus scrofa domesticus)
Target
Pig CD32a Protein
Host
Human embryonic kidney (HEK) 293 cells
Uniprot
XP_020945179.1
Shipping Conditions
Ambient
Background
CD32a (FcγRIIa) is an activating, immune-complex-binding Fc gamma receptor on myeloid cells and platelets, and characterizing its porcine counterpart matters for translational and xenotransplantation research in swine. Expressed in HEK 293 cells, this recombinant pig CD32a ectodomain is validated for SPR, BLI and ELISA, enabling cross-species comparison of antibody Fc engagement and immune-complex binding. It is a non-therapeutic reagent for research use only, prepared to in vivo grade specifications with ultra-low endotoxin. Manufactured in scalable milligram (mg) to gram (g) quantities, it gives high-throughput and bulk laboratories a dependable tool for porcine Fc receptor and antibody-effector studies.
Other Names
CD32, CD32A, FCGR2, FCGR2A, FCGRII, FCGRIIA, FCR2, FCR2A, FCRII, FCRIIA, IGFR2, IGFR2A, IGFRII, IGFRIIA, Fc gamma R2, Fc gamma R2a, Fc gamma RII, Fc gamma RIIa, Fcg R2, Fcg R2a, Fcg RII, Fcg RIIa
Specificity
The sequence of the extracellular domain of porcine CD32a (Ala 1-Gln 185) was fused with a C-terminal tag consisting of the AVI tag, TEV protease recognition sequence and a 10-His tag.
Formulation
Lyophilized from sterile PBS, pH 7.4. No preservatives or cryoprotectants have been added. To obtain a final concentration of 1 mg/ml reconstitute 0.25 mg vials with 0.25 ml water and 1.0 mg vials with 1.0 ml water.
Storage
Lyophilized proteins are stable at ambient temperature for at least 2 weeks. If the protein is not to be used immediately then the protein should be stored in lyophilized form at -20 °C for up 12 months. Once the protein has been reconstituted we recommen
Applications
SPR, BLI, ELISA
Use
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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