Human CD32a (167R) Protein - Biotin

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SKU
ICH3012B
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$1,200.00

Human CD32a (167R) Protein - Biotin

Endotoxin
<1.0 EU per mg as determined by the LAL method.
Purity
>95% monomer purity as determined by SDS-PAGE and SEC-HPLC.
Species Reactivity
Human
Target
Human CD32a (167R) protein
Host
Human embryonic kidney (HEK) 293 cells.
Uniprot
P12318
Shipping Conditions
Ambient
Background
A low-affinity activating IgG receptor, CD32a (FcgammaRIIa) carries a 167R (arginine) polymorphism that alters immune-complex engagement relative to the 167H allele. This biotinylated recombinant human CD32a (167R) protein, produced in HEK293 cells, is designed for streptavidin-based capture in SPR, BLI, and ELISA workflows and is a protein reagent rather than an antibody. Prepared to in vivo-grade, ultra-low-endotoxin specifications, it is offered in scalable milligram to gram quantities for bulk and high-throughput laboratories. The biotin tag makes it ideal for immobilized binding and kinetic studies of allele-specific Fc-receptor interactions, supplied strictly for research use only.
Other Names
Human Fc gamma RIIa, CD32A, FCGR2A, FCGRIIA, FCR2A, FCRIIA, IGFR2A, IGFRIIA
Specificity
The sequence of the extracellular domain of human CD32a (Ala 36-Ile 218) was fused with a C-terminal tag consisting of the AVI tag, TEV protease recognition sequence and a 10-His tag.
Formulation
Lyophilized from sterile PBS, pH 7.4. No preservatives or cryoprotectants have been added. To obtain a final concentration of 1 mg/ml reconstitute 0.25 mg vials with 0.25 ml water and 1.0 mg vials with 1.0 ml water.
Storage
Lyophilized proteins are stable at ambient temperature for at least 2 weeks. If the protein is not to be used immediately then the protein should be stored in lyophilized form at -20 °C for up 12 months. Once the protein has been reconstituted we recommen
Applications
SPR, BLI, ELISA
Use
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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