Bulk Human IgG1 (IB1) Isotype Control N297A

Human

IgG1

≥ 5.0 mg/ml

Human IgG1

CHO

Aggregation level ≤ 5%

ICH3001-100ml ICH3002-100ml ICH3003-100ml

P01857

Blue ice

Clone IB1 with the N297A aglycosylation mutation is a human IgG1 isotype control that eliminates Fc glycan-mediated effector activity, making it a clean negative control where effector engagement must be ruled out. IB1 N297A shares the IgG1 framework of test antibodies and supplies the background and gating reference needed in flow cytometry and functional assays, without contributing specific binding. The anti-IgG1 control clone IB1 is delivered in a low-endotoxin, in-vivo-suitable format and manufactured in scalable milligram to gram quantities for high-throughput and bulk laboratories. It is intended strictly for research use only and is non-therapeutic.

IB1

This Human IgG1 isotype control recombinant antibody has been tested against selected species' cells and tissues to assure minimal cross reactivity. Furthermore, this low endotoxin Human IgG1 antibody is suitable for in vivo applications and each lot is IMPACT I certified.

This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.

This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

Flow Cytometry, Functional Assays

Each investigator should determine their own optimal working dilution for specific applications.

Products are for research use only. Not for use in diagnostic or therapeutic procedures.