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Bulk Anti-Human CD3 (UCHT-1) Antibody
$400.00 - $2,945.00
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Bulk anti-human CD3 antibody (UCHT-1)
Endotoxin
<1.0 EU/mg as determined by the LAL method
Purity
>95% by SDS-PAGE and HPLC
Product Specific Citations
Species Reactivity
Human
Target
CD3
Concentration
≤ 2.0 mg/ml
Isotype
Mouse IgG1
Host
Mouse
Use
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
Application Notes
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing CD3. A suggested method would be to stain cells expressing CD3 with 2.0 µg per million cells in a total staining volume of 100 µl followed by Goat Anti-Mouse IgG (H&L)-R-phycoerythrin.
Each investigator should determine their own optimal working dilution for specific applications.
Applications
Flow Cytometry, Immunoprecipitation, Western Blot, IHC (Frozen), CyTOF, Functional Assays
Storage
anti-CD3 In Vivo Antibody - Low Endotoxin (UCHT-1) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Formulation
0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.
Purification Method
This monoclonal antibody was purified using Protein A
Specificity
Anti-CD3 In Vivo Antibody - Low Endotoxin (UCHT-1) recognizes Human CD3. anti-Human CD3 recognizes a (Mr 22-28 kDa) T-cell surface glycoprotein. The epitope recognized by the CD3 antibody is expressed on a constant region of the epsilon chain of the CD3 antigen/T-cell receptor complex (TCR)
Shipping Conditions
Blue ice
Background
CD3 forms the signal-transducing core of the T-cell receptor complex and is central to T-lymphocyte identity and activation. The widely used clone UCHT-1 is a mouse IgG1 monoclonal antibody recognising human CD3, validated across flow cytometry, immunoprecipitation, Western blot, frozen-section IHC, CyTOF and functional assays. Manufactured to in vivo standards, low endotoxin UCHT-1 is offered in scalable milligram (mg) to gram (g) batches for heavy-usage and high-throughput workflows. As a non-therapeutic reagent for research use only (RUO), it provides reliable, reproducible results across diverse T-cell biology and immunology applications.
Other Names
CD3 epsilon chain, CD3E, T-cell surface antigen T3/Leu-4 epsilon chain
Clone
UCHT-1
Uniprot
P07766
Buffer
ICH3001-100ml
ICH3002-100ml
ICH3003-100ml
Antigen Distribution
The CD3 antigen is expressed on approximately 60-80% of human peripheral blood lymphocytes, 20-40% of splenic lymphocytes, the majority of T-CLL and approximately 70% of T-ALL Cells.
Immunogen
Unknown
Aggregation
Aggregation level ≤ 5%
Antibodies Against The Same Target
More Information
Bio X Cell:
ICH1002 is up to 37% cheaper for industry customers than the equivalent product BE0231 from Bio X Cell. Product Benefits:
ichorbio's anti-human CD3 antibody (UCHT-1) is manufactured in a cGMP compliant, ISO Quality Standard 9001:2015 facility. ichorbio's low endotoxin antibodies have half the endotoxin of comparable antibodies from Bio X Cell at less than 1.0 EU/mg. If ichorbio's low endotoxin antibodies are not low enough we also offer ultra low endotoxin antibodies which have even less endotoxin (<0.5EU/mg) at an even higher purity (98% versus 95%). ichorbio: the best antibodies for in vivo research.
Size:ichorbio's UCHT-1 in vivo antibody is available in the following bulk sizes: 1mg, 5mg, 25mg, 50mg and 100mg ichorbio regularly manufactures multi-gram amounts of our anti-CD3 UCHT-1 clone - please contact us for pricing.
Isotype:Mouse IgG1
Other Names:CD3 epsilon chain, CD3E, T-cell surface antigen T3/Leu-4 epsilon chain
Uniprot: Immunogen:Unknown
Isotype Control:Mouse IgG1 Isotype Control for In Vivo - Low Endotoxin [HKSP] (ICH2247)
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