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Anti-Mouse CD44 (IM7) In Vivo Antibody - Low Endotoxin
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Anti-Mouse CD44 (IM7) In Vivo Antibody - Low Endotoxin
| Concentration | 0.5 mg/ml |
|---|---|
| Isotype | Rat IgG2b |
| Host | Rat |
| Use | Products are for research use only. Not for use in diagnostic or therapeutic procedures. |
| Application Notes | Each investigator should determine their own optimal working dilution for specific applications. |
| Applications | Immunoprecipitation, Western Blot, Flow Cytometry, CyTOF, IHC (Frozen), ELISA, Functional Assays |
| Storage | anti-CD44 In Vivo Antibody - Low Endotoxin (IM7) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at ï؟½80°C. Avoid Repeated Freeze Thaw Cycles. |
| Endotoxin | ≤ 1.0 EU/mg as determined by the LAL method |
| Purity | >95% by SDS-PAGE and HPLC |
| Formulation | 0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. |
| Purification Method | This monoclonal antibody was purified using Protein G |
| Specificity | Anti-CD44 In Vivo Antibody - Low Endotoxin (IM7) recognizes an epitope common to alloantigens & all isoforms of CD44, that is located between amino acids 145 and 186 |
| Species Reactivity | Mouse |
| Target | CD44 |
| Shipping Conditions | Blue ice |
| background | CD44 is a 80-95 kD glycoprotein also known as Hermes, Pgp1, H-CAM, or HUTCH. It is expressed on all leukocytes, endothelial cells, hepatocytes, and mesenchymal cells. As B and T cells become activated or progress to the memory stage, CD44 expression incre |
| Other names | Extracellular matrix receptor III, ECMR-III, GP90 lymphocyte homing/adhesion receptor, HUTCH-I, Hermes antigen, Hyaluronate receptor, Lymphocyte antigen 24, Ly-24, Phagocytic glycoprotein 1, PGP-1, Phagocytic glycoprotein I, PGP-I |
| clone | IM7 |
| UniProt | P15379 |
| Buffer | ICH3001-100ml ICH3002-100ml ICH3003-100ml |
| Antigen Distribution | The CD44 antigen is present on B-cells, monocytes, macrophages and certain subsets of thymocytes and peripheral T-cells. Mice with the Ly-24.1 allotype have high densities of CD44+ T-cells, while Ly-24.2 strains are low expressors. The antibody cross reac |
| Immunogen | Dexamethasone-induced myeloid leukemia M1 cells |
| Aggregation | Aggregation level ≤ 5% |
| Related Antibody | Anti-CD44 In Vivo Antibody - Ultra Low Endotoxin [IM7] (ICH1115UL) |
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