Mouse CD32b Protein - Biotin

Mouse

Mouse CD32b Protein

Human embryonic kidney (HEK) 293 cells

P08101

Ambient

The inhibitory receptor mouse CD32b (FcγRIIb) sets a critical threshold on immune activation and is a common focus of preclinical Fc and immune-regulation studies. Provided as a biotinylated recombinant ectodomain from HEK 293 cells, this mouse CD32b protein is optimized for oriented streptavidin capture in SPR, BLI and ELISA assays of antibody Fc binding. It is a non-therapeutic reagent for research use only, manufactured to in vivo grade with ultra-low endotoxin. Scalable milligram (mg) to gram (g) availability makes it well suited to high-throughput screening and large-volume kinetic studies of murine inhibitory Fc receptor function.

CD32, CD32B, FCGR2, FCGR2B, FCGRII, FCGRIIB, FCR2, FCR2B, FCRII, FCRIIB, IGFR2, IGFR2B, IGFRII, IGFRIIB, Fc gamma R2, Fc gamma R2b, Fc gamma RII, Fc gamma RIIb, Fcg R2, Fcg R2b, Fcg RII, Fcg RIIb

The sequence of the extracellular domain of mouse CD32b (Leu 40-Ala 217) was fused with a C-terminal tag consisting of the AVI tag, TEV protease recognition sequence and a 10-His tag.

Lyophilized from sterile PBS, pH 7.4. No preservatives or cryoprotectants have been added. To obtain a final concentration of 1 mg/ml reconstitute 0.25 mg vials with 0.25 ml water and 1.0 mg vials with 1.0 ml water.

Lyophilized proteins are stable at ambient temperature for at least 2 weeks. If the protein is not to be used immediately then the protein should be stored in lyophilized form at -20 °C for up 12 months. Once the protein has been reconstituted we recommen

SPR, BLI, ELISA

Products are for research use only. Not for use in diagnostic or therapeutic procedures.