Human FcRn heterodimer Protein (In Vivo Grade, Ultra-Low Endotoxin)

Human

Human FcRn heterodimer Protein

Human embryonic kidney (HEK) 293 cells.

P55899 / P61769

Ambient

The neonatal Fc receptor (FcRn) is the MHC-class-I-like heterodimer of an alpha chain and beta-2-microglobulin that governs IgG and albumin half-life through pH-dependent recycling, a property central to antibody pharmacokinetics. This recombinant human FcRn heterodimer is expressed in HEK 293 cells and validated for SPR, BLI and ELISA, making it a defined tool for pH-dependent IgG binding studies, Fc engineering and half-life-extension research. Supplied for research use only as an in vivo grade, ultra-low-endotoxin preparation, it is produced in scalable milligram (mg) to gram (g) quantities so bulk and high-throughput laboratories can run FcRn binding assays with reliable reproducibility.

Human FCGRT-B2M, FcRn, FCGRT & B2M, Neonatal Fc receptor

The sequence of the extracellular domain of human FCGRT (Ala 24-Ser 297) was fused with a C-terminal tag consisting of the AVI tag, TEV protease recognition sequence and a 10-His tag. This was co-transfected with the sequence of human B2M (Ile 21-Met 119) and the resulting FcRn heterodimer was purified. The full protein sequences can be downloaded from the product webpage.

Lyophilized from sterile PBS, pH 7.4. No preservatives or cryoprotectants have been added. To obtain a final concentration of 1 mg/ml reconstitute 0.25 mg vials with 0.25 ml water and 1.0 mg vials with 1.0 ml water.

Lyophilized proteins are stable at ambient temperature for at least 2 weeks. If the protein is not to be used immediately then the protein should be stored in lyophilized form at -20 °C for up 12 months. Once the protein has been reconstituted we recommen

SPR, BLI, ELISA

Products are for research use only. Not for use in diagnostic or therapeutic procedures.