Human CD32a (167H) Protein - Biotin

Human

Human CD32a (167H) Protein

Human embryonic kidney (HEK) 293 cells.

P12318

Ambient

The 167H (histidine) polymorphism of CD32a (FcgammaRIIa), a low-affinity activating IgG receptor, shapes immune-complex engagement and downstream signaling. This biotinylated recombinant human CD32a (167H) protein, produced in HEK293 cells, is intended for streptavidin-based capture in SPR, BLI, and ELISA assays and is a protein reagent rather than an antibody. Prepared to in vivo-grade, ultra-low-endotoxin standards, it is available in scalable milligram to gram quantities for bulk and high-throughput laboratories. The biotin handle suits immobilized binding and kinetic studies of allele-specific Fc-receptor interactions, with this reagent supplied strictly for research use only.

Human Fc gamma RIIa, CD32A, FCGR2A, FCGRIIA, FCR2A, FCRIIA, IGFR2A, IGFRIIA

The sequence of the extracellular domain of human CD32a (Ala 36-Ile 218) was fused with a C-terminal tag consisting of the AVI tag, TEV protease recognition sequence and a 10-His tag.

Lyophilized from sterile PBS, pH 7.4. No preservatives or cryoprotectants have been added. To obtain a final concentration of 1 mg/ml reconstitute 0.25 mg vials with 0.25 ml water and 1.0 mg vials with 1.0 ml water.

Lyophilized proteins are stable at ambient temperature for at least 2 weeks. If the protein is not to be used immediately then the protein should be stored in lyophilized form at -20 °C for up 12 months. Once the protein has been reconstituted we recommen

SPR, BLI, ELISA

Products are for research use only. Not for use in diagnostic or therapeutic procedures.