Anti-Mouse CTLA-4 (9D9) In Vivo Antibody, Low Endotoxin

Bulk anti-CTLA-4 In Vivo Antibody - Low Endotoxin (9D9)

Bio X Cell:

ICH1096 is up to 19% cheaper for academia & non-profits and up to 47% cheaper for industry than the equivalent product from Bio X Cell (BE0164). ICH1096UL (ultra-low endotoxin) is up to 7% cheaper for academia and up to 39% cheaper for industry than BP0164.

Product Benefits:

ichorbio's anti-CTLA-4 In Vivo Antibody - Low Endotoxin (9D9) is manufactured in a cGMP compliant, ISO Quality Standard 9001:2015 facility. ichorbio's low endotoxin antibodies have half the endotoxin of comparable antibodies from Bio X Cell at less than 1.0 EU/mg. If ichorbio's low endotoxin antibodies are not low enough we also offer ultra low endotoxin antibodies which have even less endotoxin (<0.5EU/mg) at an even higher purity (98% versus 95%). ichorbio: the best antibodies for in vivo research.

Target:

CTLA-4

Clone:

9D9

Size:

ichorbio's 9D9 in vivo antibody is available in the following bulk sizes: 1mg, 5mg, 25mg, 50mg and 100mg ichorbio regularly manufactures multi-gram amounts of our anti-CTLA-4 9D9 clone - please contact us for pricing.

Isotype:

Mouse IgG2b

Other Names:

Ctla4, Cytotoxic T-lymphocyte protein 4, CD152

Uniprot:

P09793

Host:

Mouse

Species Reactivity:

Mouse

Specificity:

Anti-CTLA-4 In Vivo Antibody - Low Endotoxin (9D9) recognizes an epitope on Mouse CTLA-4

Purification Method:

This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Antigen Distribution:

Activated T cells

Background:

Negative regulator of T cell activation

Immunogen:

Unknown

Concentration:

1.0 - 5.0 mg/ml

Formulation:

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and Azide free.

Purity:

>95% by SDS-PAGE and HPLC

>98% by SDS-PAGE and HPLC

Endotoxin:

≤ 1.0 EU/mg as determined by the LAL method

≤ 0.75 EU/mg as determined by the LAL method

Aggregation:

Aggregation level ≤ 5%

Aggregation level ≤ 1%

IMPACT Pathogen Test:

We use the IMPACT test generated by IDEXX Laboratories to guarantee our Ultra Low Endotoxin antibodies are pathogen free. Our mouse antibodies are tested for: Mycoplasma spp. Mycoplasma pulmonis Sendai virus Mouse hepatitis virus Pneumonia virus of mice Minute virus of mice Mouse parvovirus (MPV1-5) Theiler's murine encephalomyelitis virus Murine norovirus Reovirus 3 Mouse rotavirus Ectromelia virus Lymphocytic choriomeningitis virus Polyoma virus Lactate dehydrogenase-elevating virus Mouse adenovirus (MAD1, MAD2) Mouse cytomegalovirus K virus Mouse thymic virus Hantaan virus Corynebacterium bovis Corynebacterium spp. (HAC2)

Storage:

This antibody is stable for at least 4 weeks when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 20°C or -80°C. Avoid repeated freeze thaw cycles.

Applications:

Western Blot, Functional Assays

in vivo

We recommend using between 50-250µg per mouse when performing in vivo research using ichorbio’s CTLA-4 low endotoxin antibody clone 9D9. This range is based off the most recent publication data using the 9D9 clone in vivo.

Application Notes:

Each investigator should determine their own optimal working dilution for specific applications.

Use:

Products are for research use only.

Isotype Control:

Mouse IgG2b Isotype Control for In Vivo - Low Endotoxin [MPC-11] (ICH2250)

Antibodies against the same target:

Anti-CTLA-4 In Vivo Antibody - Low Endotoxin [9H10] (ICH1084), Anti-CTLA-4 In Vivo Antibody - Low Endotoxin [UC10-4F10-11] (ICH1085), Anti-CTLA-4 In Vivo Antibody - Ultra Low Endotoxin [9H10] (ICH1084UL)

Alternative Names: