Technique Articles

1. Introduction

Dendritic cells (DCs) are crucial immune system components, acting as primary antigen-presenting cells that bridge the innate and adaptive immune responses [1]. Depleting dendritic cells in vivo is a standard experimental technique to study...

1. Understanding CD8+ T Cell Depletion

The fall or removal of CD8+ T cells, a form of T cell depletion, leads to the recognition and eradicating of various abnormal cells, virally infected cells, and tumor cells because CD8+ T cells are essential components of the immune defense system [1]. There are several ways to eliminate T cells, but antibody-based depletion has become a breakthrough in the respective...

1. Introduction

The NK (Natural killer) cells comprise various vital elements of the innate immune system. They significantly fight against malignant cells and viral infections. Scientists can discover the particular contributions of NK cell depletion in...

1. Introduction

T cells play a crucial role in advancing the broad field of immunological research, particularly CD4+ T cells. A practical method is T cell depletion in vivo, which has effectively stimulated numerous immune response and their applications...

 1. Introduction

Researchers used a complex method like neutrophil depletion to regulate the immune responses in different trial cases [1]. The review will explore insight into neutrophil depletion, including its policies and importance and the...

Introduction

Immune...

Purpose

This protocol...

Objective

This...

Solutions and Buffers

Lysis Buffers

These buffers can be stored at 4°C for several weeks or aliquoted and stored at -20°C for up to a year.

1. NP-40 Buffer

   - 150 mM NaCl

   - 1.0% NP-40 (can be replaced with 0.1% Triton X-100)

   - 50 mM Tris-HCl, pH 8.0

   - Protease inhibitors

2. RIPA Buffer

   - 150 mM...

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Basic things to remember when injecting mice

1. Use a new needle for each injected animal. Consider needle-free injectors if possible...