Protocols

1. Introduction

Dendritic cells (DCs) are crucial immune system components, acting as primary antigen-presenting cells that bridge the innate and adaptive immune responses [1]. Depleting dendritic cells in vivo is a standard experimental technique to study...

1. Understanding CD8+ T Cell Depletion

The fall or removal of CD8+ T cells, a form of T cell depletion, leads to the recognition and eradicating of various abnormal cells, virally infected cells, and tumor cells because CD8+ T cells are essential components of the immune defense system [1]. There are several ways to eliminate T cells, but antibody-based depletion has become a breakthrough in the respective...

1. Introduction

The NK (Natural killer) cells comprise various vital elements of the innate immune system. They significantly fight against malignant cells and viral infections. Scientists can discover the particular contributions of NK cell depletion in...

1. Introduction

T cells play a crucial role in advancing the broad field of immunological research, particularly CD4+ T cells. A practical method is T cell depletion in vivo, which has effectively stimulated numerous immune response and their applications...

 1. Introduction

Researchers used a complex method like neutrophil depletion to regulate the immune responses in different trial cases [1]. The review will explore insight into neutrophil depletion, including its policies and importance and the...

Purpose

This protocol...

Objective

This...

Solutions and Buffers

Lysis Buffers

These buffers can be stored at 4°C for several weeks or aliquoted and stored at -20°C for up to a year.

1. NP-40 Buffer

   - 150 mM NaCl

   - 1.0% NP-40 (can be replaced with 0.1% Triton X-100)

   - 50 mM Tris-HCl, pH 8.0

   - Protease inhibitors

2. RIPA Buffer

   - 150 mM...

Exosomal PD-L1 (exoPD-L1) has recently received significant attention as a biomarker predicting immunotherapeutic responses involving the PD1/PD-L1 pathway. However, current technologies for exosomal analysis rely primarily on bulk measurements that do not consider the heterogeneity found within exosomal subpopulations.

A new study presents a nanoscale cytometry platform called NanoEPIC, enabling phenotypic sorting and exoPD-L1 profiling from...

Ichorbio has created a series of documents that we hope will be really helpful for organising your experiments and meetings in an around the laboratory. Our laboratory planning documents are free to download – they’re available individually or as a bulk pack of 12 pages. 

It may be that you won’t use these pages day to day as you’ll probably have a notebook for all your lab work. However we hope that the digital files will give you some...