Anti-Mouse CD49d In Vivo Antibody (PS/2) – Low Endotoxin [ICH1200]

ichorbio’s anti-Mouse CD49d In Vivo Antibody (PS/2) – Low Endotoxin [ICH1200] is manufactured in a cGMP compliant, ISO Quality Standard 9001:2015 facility. ichorbio’s low endotoxin antibodies have half the endotoxin of comparable antibodies from our competitors at less than 1.0 EU/mg. If ichorbio’s low endotoxin antibodies are not low enough we also offer ultra low endotoxin antibodies which have even less endotoxin (<0.75EU/mg) at an even higher purity (98% versus 95%). ichorbio: the best antibodies for in vivo research.

CD49d

PS/2

ichorbio’s PS/2 in vivo antibody is available in the following bulk sizes: 5mg, 25mg, 50mg and 100mg. ichorbio regularly manufactures multi-gram amounts of our anti-mouse CD49d PS/2 clone – please contact us for pricing.

Rat IgG2b kappa

VLA-4α, ITGA4, Integrin α4

Rat

Mouse, Human

PS/2 activity is directed against mouse CD49d and is cross reactive against human CD49d.

CD49d is expressed on T cells, B cells, NK , dendritic cells, thymocytes, monocytes, eosinophils, mast cells.

Hybridoma supernatants were screened by cell adhesion assay and cells producing blocking antibodies were cloned. Adhesion is blocked in a dose dependent manner when PS/2 is used with P815 and +/+ 2.4 stromal cells. 70Z/3 cells are also sensitive to PS/2 inhibition. PS/2 is known to block binding of CD49d to its ligands. Lymphocyte production is completely blocked when PS/2 is included in Whitlock-Witte culture.

PS/2 was generated by immunizing Fisher rats with P815 cells and subsequently fusing the spleen cells with Sp2/0.

1.0 – 5.0 mg/ml

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and Azide free.

>95% by SDS-PAGE and HPLC

>98% by SDS-PAGE and HPLC

<1.0 EU/mg as determined by the LAL method

≤ 0.75 EU/mg as determined by the LAL method

Aggregation level ≤ 5%

Aggregation level ≤ 1%

Anti-Mouse CD49d In Vivo Antibody (PS/2) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

Depletion, Flow Cytometry, Immunofluorescence, Immunohistochemistry

Each investigator should determine their own optimal working dilution for specific applications.

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

Rat IgG2b In Vivo Isotype Control – Low Endotoxin (1-2) [ICH2243]