Anti-Mouse CD49d (PS/2) In Vivo Antibody - Low Endotoxin

Anti-Mouse CD49d In Vivo Antibody (PS/2)

Product Benefits:

ichorbio's anti-Mouse CD49d In Vivo Antibody (PS/2) - Low Endotoxin [ICH1200] is manufactured in a cGMP compliant, ISO Quality Standard 9001:2015 facility. ichorbio's low endotoxin antibodies have half the endotoxin of comparable antibodies from Bio X Cell at less than 1.0 EU/mg. If ichorbio's low endotoxin antibodies are not low enough we also offer ultra low endotoxin antibodies which have even less endotoxin (<0.5EU/mg) at an even higher purity (98% versus 95%). ichorbio: the best antibodies for in vivo research.

Target:

CD49d

Clone:

PS/2

Size:

ichorbio's PS/2 in vivo antibody is available in the following bulk sizes: 5mg, 25mg, 50mg and 100mg. ichorbio regularly manufactures multi-gram amounts of our anti-mouse CD49d PS/2 clone - please contact us for pricing.

Isotype:

Rat IgG2b kappa

Other Names:

VLA-4α, ITGA4, Integrin α4

Host:

Rat

Species Reactivity:

Mouse, Human

Specificity:

PS/2 activity is directed against mouse CD49d and is cross reactive against human CD49d.

Antigen Distribution:

CD49d is expressed on T cells, B cells, NK , dendritic cells, thymocytes, monocytes, eosinophils, mast cells.

Background:

Hybridoma supernatants were screened by cell adhesion assay and cells producing blocking antibodies were cloned. Adhesion is blocked in a dose dependent manner when PS/2 is used with P815 and +/+ 2.4 stromal cells. 70Z/3 cells are also sensitive to PS/2 inhibition. PS/2 is known to block binding of CD49d to its ligands. Lymphocyte production is completely blocked when PS/2 is included in Whitlock-Witte culture.

Immunogen:

PS/2 was generated by immunizing Fisher rats with P815 cells and subsequently fusing the spleen cells with Sp2/0.

Concentration:

1.0 - 5.0 mg/ml

Formulation:

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and Azide free.

Purity:

>95% by SDS-PAGE and HPLC

>98% by SDS-PAGE and HPLC

Endotoxin:

<1.0 EU/mg as determined by the LAL method

≤ 0.75 EU/mg as determined by the LAL method

Aggregation:

Aggregation level ≤ 5%

Aggregation level ≤ 1%

IMPACT Pathogen Test:

We use the IMPACT test generated by IDEXX Laboratories to guarantee our Ultra Low Endotoxin antibodies are pathogen free. Our mouse antibodies are tested for: Mycoplasma spp., Mycoplasma pulmonis, Sendai virus, Mouse hepatitis virus, Pneumonia virus of mice, Minute virus of mice, Mouse parvovirus (MPV1-5), Theiler's murine encephalomyelitis virus, Murine norovirus, Reovirus 3, Mouse rotavirus, Ectromelia virus, Lymphocytic choriomeningitis virus, Polyoma virus, Lactate dehydrogenase-elevating virus, Mouse adenovirus (MAD1, MAD2), Mouse cytomegalovirus K virus, Mouse thymic virus, Hantaan virus, Corynebacterium bovis, Corynebacterium spp. (HAC2)

Storage:

Anti-Mouse CD49d In Vivo Antibody (PS/2) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

Applications:

Depletion, Flow Cytometry, Immunofluorescence, Immunohistochemistry

Application Notes: Each investigator should determine their own optimal working dilution for specific applications.

Use:

Products are for research use only.

Isotype Control:

Rat IgG2b In Vivo Isotype Control – Low Endotoxin (1-2) [ICH2243]