Mouse IgG2a Isotype Control - Extremely Low Endotoxin

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SKU
ICH2259
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$937.00 - $2,530.00
Delivery Time : 4 weeks

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Mouse IgG2a Isotype Control - Extremely Low Endotoxin

Endotoxin
<0.05 EU/mg as determined by the LAL method
Purity
>95% by SDS-PAGE
Concentration
1.0 - 5.0 mg/ml
Host
CHO
Buffer
ICH3002
Background
This mouse IgG2a isotype control is provided at extremely low endotoxin levels, delivering a subclass-matched negative reference for demanding in vivo and functional immunology work. With no relevant target, it defines the background signal and gating thresholds that distinguish genuine antigen engagement from scaffold-driven signal in flow cytometry and functional assays. The reagent reproduces the Fc behaviour of mouse IgG2a test antibodies, isolating non-specific effects. It is validated for in vivo administration and manufactured in scalable milligram to gram quantities for bulk and high-throughput laboratories. This is a research-use-only, non-therapeutic product.
Purification Method
This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Storage
This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
Applications
Flow Cytometry, Functional Assays
Application Notes
Each investigator should determine their own optimal working dilution for specific applications.
Use
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
Delivery Time
4 weeks

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