Mouse FcRn heterodimer Protein (In Vivo Grade, Ultra-Low Endotoxin)

Mouse

Mouse FcRn heterodimer Protein

Human embryonic kidney (HEK) 293 cells

Q61559 / P01887

Ambient

In mice, FcRn controls the half-life of IgG and albumin through pH-dependent recycling, a mechanism widely exploited when optimizing antibody pharmacokinetics in preclinical models. This recombinant mouse FcRn heterodimer, formed from the alpha chain and beta-2-microglobulin, is expressed in HEK 293 cells and validated for SPR, BLI and ELISA to support pH-dependent IgG binding studies. It is supplied for research use only in an in vivo grade, ultra-low-endotoxin format and produced in scalable milligram (mg) to gram (g) quantities, giving bulk and high-throughput laboratories a dependable murine FcRn reagent for pharmacokinetics and Fc engineering research.

FcRn, FCGRT, FCGRT & B2M, FCGRT and B2M, Neonatal Fc receptor, Neonatal receptor, Brambell receptor

The sequence of the extracellular domain of mouse FCGRT (Ser 22-Ser 297) was fused with a C-terminal tag consisting of the AVI tag, TEV protease recognition sequence and a 10-His tag. This was co-transfected with the sequence of mouse B2M (Ile 21-Met 119) and the resulting FcRn heterodimer was purified. The full protein sequences can be downloaded from the product webpage.

Lyophilized from sterile PBS, pH 7.4. No preservatives or cryoprotectants have been added. To obtain a final concentration of 1 mg/ml reconstitute 0.25 mg vials with 0.25 ml water and 1.0 mg vials with 1.0 ml water.

Lyophilized proteins are stable at ambient temperature for at least 2 weeks. If the protein is not to be used immediately then the protein should be stored in lyophilized form at -20 °C for up 12 months. Once the protein has been reconstituted we recommen

SPR, BLI, ELISA

Products are for research use only. Not for use in diagnostic or therapeutic procedures.