Bulk anti-Human CXCR4 Antibody (12G5)
ichorbio’s bulk anti-Human CXCR4 Antibody (12G5) is manufactured in a cGMP compliant, ISO Quality Standard 9001:2015 facility.
Fusin, LESTR, CD184
ichorbio’s anti-Human CXCR4 antibody is available in the following bulk sizes: 5mg, 25mg, 50mg and 100mg. ichorbio regularly manufactures multi-gram amounts of our anti-CXCR4 clone 12G5 – please contact us for pricing.
Mouse IgG2a kappa
12G5 activity is directed against human CXCR4 (CD184; Fusin).
This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
CXCR4 is expressed in various organs including ovary, bone marrow, kidney, lung, small intestine, spleen, lymph nodes, brain, stomach, liver, thymus, heart, and pancreas as well as on the surface of endothelial mature and precursor cells and pericytes.
12G5 binds specifically to both human and nonhuman cells that express recombinant CXCR4. 12G5 inhibits CD4-independent infection by some HIV-2 isolates, and preincubating cells with 12G5 abolishes syncytium formation. HIV-2/vcp-infected cells display a marked and selective reduction in 12G5 binding. 12G5 also inhibits induction of cell-to-cell fusion of CXCR4+ RD/CD4 cells by HIV-1 and HIV-2 strains.
12G5 was produced by immunizing Balb/c mice with CP-MAC-infected Sup-T1 cells. Hybridomas were generated and screened for the ability to inhibit CP-MAC-induced syncytium induction on Sup-T1 cells.
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (PBS) pH 7.2 – 7.4, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and azide free
>95% by SDS-PAGE and HPLC
>98% by SDS-PAGE and HPLC
≤ 1.0 EU/mg as determined by the LAL method
≤ 0.75 EU/mg as determined by the LAL method
Aggregation level ≤ 5%
Aggregation level ≤ 1%
This antibody is stable for at least 4 weeks when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 20°C or -80°C. Avoid repeated freeze thaw cycles.
Blocking, Flow Cytometry, Immunocytochemistry, Immunofluorescence Microscopy, Immunohistochemistry, Neutralization, Western Blotting
Each investigator should determine their own optimal working dilution for specific applications.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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