Anti-Mouse CD317 (927) In Vivo Antibody - Low Endotoxin

Anti-Mouse CD317 In Vivo Antibody (927) - Low Endotoxin

Product Benefits:

ichorbio's anti-Mouse CD317 In Vivo Antibody (927) - Low Endotoxin is manufactured in a cGMP compliant, ISO Quality Standard 9001:2015 facility.

ichorbio's low endotoxin antibodies have half the endotoxin of comparable antibodies from Bio X Cell at less than 1.0 EU/mg.

If ichorbio's low endotoxin antibodies are not low enough we also offer ultra low endotoxin antibodies which have even less endotoxin (<0.5 EU/mg) at an even higher purity (98% versus 95%).

ichorbio: the best antibodies for in vivo research.

Target:

CD317

Clone:

927

Size:

ichorbio's 927 in vivo antibody is available in the following bulk sizes: 5mg, 25mg, 50mg and 100mg. ichorbio regularly manufactures multi-gram amounts of our anti-mouse CD317 927 clone - please contact us for pricing.

Isotype:

Rat IgG2a kappa

Host:

Rat

Species Reactivity:

Mouse

Specificity:

Clone 927 activity is directed against murine CD317 (BST2; PDCA-1).

Antigen Distribution:

Murine CD317 is expressed by IFN-producing cells in naïve mouse spleen and by a wide variety of cell lines including T cells, mast cells, B cells, fibroblast cells, and pluripotent embryonal carcinoma cells. Additionally, mice challenged by influenza or other stimuli (CpG, LPS, murine CMV, poly(I:C), and imiquimod) express CD317 in DC and other myeloid cells as well as T cells, B cells, NKT cells, and some NK cells. CD317 is also expressed on CD138+ plasma cells in naïve mice and is upregulated by viral stimulation..

Immunogen:

Mouse plasmacytoid dendritic cells.

Concentration:

1.0 - 5.0 mg/ml

Formulation:

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and Azide free.

Purity:

>95% by SDS-PAGE and HPLC

>98% by SDS-PAGE and HPLC

Endotoxin:

<1.0 EU/mg as determined by the LAL method

≤ 0.5 EU/mg as determined by the LAL method

Aggregation:

Aggregation level ≤ 5%

Aggregation level ≤ 1%

IMPACT Pathogen Test:

We use the IMPACT test generated by IDEXX Laboratories to guarantee our Ultra Low Endotoxin antibodies are pathogen free. Our mouse antibodies are tested for: Mycoplasma spp., Mycoplasma pulmonis, Sendai virus, Mouse hepatitis virus, Pneumonia virus of mice, Minute virus of mice, Mouse parvovirus (MPV1-5), Theiler's murine encephalomyelitis virus, Murine norovirus, Reovirus 3, Mouse rotavirus, Ectromelia virus, Lymphocytic choriomeningitis virus, Polyoma virus, Lactate dehydrogenase-elevating virus, Mouse adenovirus (MAD1, MAD2), Mouse cytomegalovirus K virus, Mouse thymic virus, Hantaan virus, Corynebacterium bovis, Corynebacterium spp. (HAC2)

Storage:

Anti-Mouse CD317 In Vivo Antibody (927) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

Applications:

Depletion, Functional Assays, Flow Cytometry, Immunocytochemistry, Immunofluorescence Microscopy

Application Notes:

Each investigator should determine their own optimal working dilution for specific applications.

Use:

Products are for research use only.