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Pig CD64 Protein (In Vivo Grade, Ultra-Low Endotoxin)
Pig CD64 Protein (In Vivo Grade, Ultra-Low Endotoxin)
Endotoxin
<1.0 EU per mg as determined by the LAL method.
Purity
>95% monomer purity as determined by SDS-PAGE and SEC-HPLC.
Species Reactivity
Pig (sus scrofa domesticus)
Target
Pig CD64 Protein
Host
Human embryonic kidney (HEK) 293 cells
Uniprot
Q461Q0
Shipping Conditions
Ambient
Background
CD64 (FcγRI) is the sole high-affinity Fc gamma receptor for monomeric IgG and a key driver of myeloid activation, and porcine versions are increasingly important as the pig becomes a translational and xenotransplantation model. This recombinant pig CD64 ectodomain is expressed in HEK 293 cells and validated for SPR, BLI and ELISA, supporting cross-species Fc binding studies and antibody characterization in swine models. The protein is supplied for research use only in an in vivo grade, ultra-low-endotoxin format and is produced in scalable milligram (mg) to gram (g) quantities, providing bulk and high-throughput laboratories with a reproducible porcine Fc receptor reagent.
Other Names
CD64, CD64A, FCGR1, FCGRI, FCGR1A, FCGRIA, FCR1, FCRI, IGFR1, IGFRI, Fc gamma R1, Fc gamma RI, Fcg R1, Fcg RI
Specificity
The sequence of the extracellular domain of porcine CD64 (Gln 16-Gly 282) was fused with a C-terminal tag consisting of the AVI tag, TEV protease recognition sequence and a 10-His tag.
Formulation
Lyophilized from sterile PBS, pH 7.4. No preservatives or cryoprotectants have been added. To obtain a final concentration of 1 mg/ml reconstitute 0.25 mg vials with 0.25 ml water and 1.0 mg vials with 1.0 ml water.
Storage
Lyophilized proteins are stable at ambient temperature for at least 2 weeks. If the protein is not to be used immediately then the protein should be stored in lyophilized form at -20 °C for up 12 months. Once the protein has been reconstituted we recommen
Applications
SPR, BLI, ELISA
Use
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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