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Anti-CD3e (500A2) In Vivo Antibody - Low Endotoxin
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Anti-CD3e (500A2) In Vivo Antibody - Low Endotoxin
| Concentration | 1.0 mg/ml |
|---|---|
| Isotype | Syrian Hamster IgG2a Kappa |
| Host | Syrian Hamster |
| Use | Products are for research use only. Not for use in diagnostic or therapeutic procedures. |
| Application Notes | Functional Assay: Binding of Hamster Anti-Mouse CD3_ (Clone 500 A2) to CD3 initiates the intracellular biochemical pathway resulting in cellular activation and proliferation. Each investigator should determine their own optimal working dilution for specif |
| Applications | Functional Assays, Flow Cytometry, IHC (Frozen) |
| Storage | anti-CD3e In Vivo Antibody - Low Endotoxin (500A2) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at ï؟½80°C. Avoid Repeated Freeze Thaw Cycles. |
| Endotoxin | <1.0 EU/mg as determined by the LAL method |
| Purity | >95% by SDS-PAGE and HPLC |
| Formulation | 0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. |
| Purification Method | This monoclonal antibody was purified using Protein A |
| Specificity | Anti-CD3e In Vivo Antibody - Low Endotoxin (500A2) recognizes an epitope on Mouse CD3_ |
| Species Reactivity | Mouse |
| Target | CD3e |
| Shipping Conditions | Blue ice |
| background | CD3 subunits gamma, delta and epsilon are required for proper assembly, trafficking and surface expression of the TCR complex. |
| Other names | T3/Leu-4 epsilon chain, CD3 epsilon chain, CD3e |
| clone | 500A2 |
| UniProt | P22646 |
| Buffer | ICH3001-100ml ICH3002-100ml ICH3003-100ml |
| Antigen Distribution | The CD3 complex is present on thymocytes and mature T-lymphocytes of all mouse strains tested. |
| Immunogen | Mouse T-cell receptor |
| Aggregation | Aggregation level ≤ 5% |
| Antibodies against the same target | Anti-CD3e In Vivo Antibody - Ultra Low Endotoxin [145-2C11] (ICH1041UL), Anti-CD3e In Vivo Antibody - Low Endotoxin [145-2C11] (ICH1041) |
| Related Antibody | Anti-CD3e In Vivo Antibody - Ultra Low Endotoxin [500A2] (ICH1040UL) |
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