Anti-ICAM-1 (15.2) In Vivo Antibody - Low Endotoxin

Human, Mouse, Rat

ICAM-1

>1mg/ml

Mouse IgG1

Mouse

Anti-Mouse ICAM-1 (BE29G1) In Vivo Antibody - Low Endotoxin (ICH1101), Bersanlimab Biosimilar - Research Grade (ICH5394), Enlimomab Biosimilar - Research Grade (ICH5525)

Aggregation level ≤ 5%

Human infant thymocytes and Sezary cells

The CD54 antigen is a membrane glycoprotein with a wide tissue distribution that includes vascular endothelium and many cells of the immune system. CD54 is weakly expressed on resting peripheral blood lymphocytes. Upon activation by mitogens, the CD54 ant

ICH3001-100ml ICH3002-100ml ICH3003-100ml

P05362

Blue ice

Clone 15.2 is a mouse monoclonal antibody (isotype IgG1) recognising ICAM-1 (CD54), historically characterised against the human antigen; the listed Human/Mouse/Rat reactivity should be confirmed for the user's species before functional work. As a purified mouse IgG1, it is well suited to adhesion- and ligand-blocking studies in vitro and in vivo rather than to depletion, since IgG1 is a weak effector isotype in mouse systems. The antibody has documented utility across IHC on FFPE tissue, flow cytometry, immunoprecipitation and Western blot, supporting both target validation and functional readouts. This in-vivo grade preparation is manufactured to low endotoxin (research grade <1 EU/mg; ultra-low <0.5 EU/mg) and supplied in bulk milligram-to-gram quantities, making it appropriate for repeat-dosing animal studies where endotoxin contamination would otherwise confound inflammatory and leukocyte-trafficking endpoints. It is provided for research use only. UniProt P05362. ICAM-1 (intercellular adhesion molecule 1, CD54) is a type I transmembrane glycoprotein of the immunoglobulin superfamily, comprising five extracellular Ig-like domains. It is expressed at low levels on endothelium, epithelium and leukocytes and is strongly upregulated by inflammatory cytokines such as TNF, IL-1 and IFN-gamma. Its principal ligands are the beta-2 integrins LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18) on leukocytes. Through these interactions ICAM-1 mediates firm adhesion, arrest and transendothelial migration of leukocytes during inflammation, and contributes to formation of the immunological synapse between antigen-presenting cells and T cells. ICAM-1 also serves as a receptor for rhinovirus and for Plasmodium falciparum-infected erythrocytes. Because of its central role in leukocyte trafficking and immune activation, ICAM-1 is a widely studied target in inflammation, autoimmunity, ischaemia-reperfusion injury and tumour biology.

Intercellular adhesion molecule 1, ICAM1, Major group rhinovirus receptor, CD54, 15 2, 15-2, 152

15.2

Mouse anti-Human Inter-Cellular Adhesion Molecule 1 (ICAM-1) (Clone 15.2) recognizes an epitope on Human ICAM-1

This monoclonal antibody was purified using Protein A

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.

This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.

IHC (FFPE), Flow Cytometry, Immunoprecipitation, Western Blot

Each investigator should determine their own optimal working dilution for specific applications.

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

Q: Is clone 15.2 suitable for in vivo ICAM-1 blocking?

A: Yes, 15.2 is supplied in a low-endotoxin, in vivo-grade format suitable for ICAM-1 blocking and adhesion studies as well as in vitro work.

Q: Can I order clone 15.2 anti-ICAM-1 in bulk?

A: Yes, it is produced in scalable milligram to gram quantities for bulk and high-throughput in vivo experiments.

Q: What species does clone 15.2 anti-ICAM-1 recognise?

A: It is an anti-ICAM-1 (CD54) monoclonal antibody with reactivity reported for human, mouse and rat, used in adhesion studies; for research use only.