Bulk anti-ICAM-1 antibody clone 15.2
ichorbio’s anti-ICAM-1 In Vivo Antibody – Low Endotoxin (15.2) [ICH1027] is manufactured in a cGMP compliant, ISO Quality Standard 9001:2015 facility. ichorbio’s low endotoxin antibodies have half the endotoxin of comparable antibodies from our competitors at less than 1.0 EU/mg. If ichorbio’s low endotoxin antibodies are not low enough we also offer ultra low endotoxin antibodies which have even less endotoxin (<0.75EU/mg) at an even higher purity (98% versus 95%). ichorbio: the best antibodies for in vivo research.
Intercellular adhesion molecule 1, ICAM1, Major group rhinovirus receptor, CD54
Human, Mouse, Rat
Mouse anti-ICAM-1 in vivo antibody – low endotoxin (15.2) recognizes an epitope on Human ICAM-1
This bulk anti-ICAM-1 antibody clone 15.2 was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and Azide free.
>95% by SDS-PAGE and HPLC
>98% by SDS-PAGE and HPLC
≤ 1.0 EU/mg as determined by the LAL method
≤ 0.75 EU/mg as determined by the LAL method
Aggregation level ≤ 5%
Aggregation level ≤ 1%
Anti-ICAM-1 In Vivo Antibody – Low Endotoxin (15.2) is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
IHC (FFPE), Flow Cytometry, Immunoprecipitation, Western Blot
Each investigator should determine their own optimal working dilution for specific applications.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
Immunofluorescence analysis of paraffin-embedded breast cancer tissue section labeling ICAM-1 (1:100) overnight at 4˚, followed by goat anti-mouse IgG H&L (Alexa Fluor ® 647-red) secondary antibody (1:500 dilution). MDA-MB-231 cells were injected via mouse nipple and grown for 5 weeks. The nuclear counter stain is DAPI (blue). Image was acquired on a Nikon A1R microscope system at 4x magnification (first image) or 60x magnification (second image).