Anti-Mouse CD24 In Vivo Antibody (M1/69) – Low Endotoxin [ICH1192]

ichorbio’s anti-Mouse CD24 In Vivo Antibody (M1/69) – Low Endotoxin [ICH1192] is manufactured in a cGMP compliant, ISO Quality Standard 9001:2015 facility. ichorbio’s low endotoxin antibodies have half the endotoxin of comparable antibodies from our competitors at less than 1.0 EU/mg. If ichorbio’s low endotoxin antibodies are not low enough we also offer ultra low endotoxin antibodies which have even less endotoxin (<0.75EU/mg) at an even higher purity (98% versus 95%). ichorbio: the best antibodies for in vivo research.

CD24

M1/69

ichorbio’s M1/69 in vivo antibody is available in the following bulk sizes: 5mg, 25mg, 50mg and 100mg. ichorbio regularly manufactures multi-gram amounts of our anti-mouse CD24 M1/69 clone – please contact us for pricing.

IgG2b kappa

Heat Stable Antigen, Nectadrin, Ly-52

Rat

Mouse

Anti-CD24 antibody (clone M1/69) activity is directed against mouse CD24, also known as Heat Stable Antigen (HSA) or Ly-52.

CD24 is expressed on B cells, T cells, neutrophils, eosinophils, macrophages, neural cells, ganglion cells, keratinocytes, muscle cells, pancreas cells, lymphocytes, granulocytes, epithelial cells, thymocytes, monocytes, erythrocytes, dendritic cells and is overexpressed in many cancers. Expression varies during T and B cell differentiation. Peripheral T cells are mainly negative.

Mouse CD24 is a small 27 amino acid sialoglycoprotein that is anchored to plasma membranes via a glycosyl-phosphatidylinositol linker. CD24 is widely distributed and plays a role in many diverse functions including adaptive immunity, inflammation, autoimmunity, and cancer. CD24 modulates growth and differentiation signals to granulocytes and B cells, is required for homeostatic cell renewal, binds to P-selectin on activated endothelial cells, plays a role in cell adhesion, lymphocyte proliferation for homeostatic purposes4, lymphocyte costimulation via CD28-independent pathways as well as a crucial role in cell selection and maturation during hematopoiesis.

M1/69 was generated by fusing the spleen cells of a DA rat immunized with B10 mouse spleen cells enriched for T cells with cells from a nonsecreting mouse myeloma line (NSI)

1.0 – 5.0 mg/ml

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and Azide free.

>95% by SDS-PAGE and HPLC

>98% by SDS-PAGE and HPLC

<1.0 EU/mg as determined by the LAL method

≤ 0.75 EU/mg as determined by the LAL method

Aggregation level ≤ 5%

Aggregation level ≤ 1%

Anti-Mouse CD24 In Vivo Antibody (M1/69) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

Inhibitor, ELISA Indirect, Flow Cytometry, Immunofluorescence, IF Microscopy, Immunohistochemistry, Immunoprecipitation, Western Blot

Each investigator should determine their own optimal working dilution for specific applications.

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

Rat IgG2b Isotype Control for In Vivo – Low Endotoxin