Anti-Mouse CD105 In Vivo Antibody (MJ7/18) – Low Endotoxin [ICH1191]

ichorbio’s anti-Mouse CD105 In Vivo Antibody (MJ7/18) – Low Endotoxin [ICH1191] is manufactured in a cGMP compliant, ISO Quality Standard 9001:2015 facility. ichorbio’s low endotoxin antibodies have half the endotoxin of comparable antibodies from our competitors at less than 1.0 EU/mg. If ichorbio’s low endotoxin antibodies are not low enough we also offer ultra low endotoxin antibodies which have even less endotoxin (<0.75EU/mg) at an even higher purity (98% versus 95%). ichorbio: the best antibodies for in vivo research.

CD105

MJ7/18

ichorbio’s MJ7/18 in vivo antibody is available in the following bulk sizes: 5mg, 25mg, 50mg and 100mg. ichorbio regularly manufactures multi-gram amounts of our anti-mouse CD105 MJ7/18 clone – please contact us for pricing.

IgG2a kappa

Endoglin, Ancillary TGF-beta Receptor

Rat

Mouse

MJ7/18 activity is directed against mouse CD105 (endoglin).

CD105 is alternatively spliced, resulting in both long (L-endoglin) and short (S-endoglin) forms. The predominant isoform, L-endoglin, promotes the proliferation and migration of endothelial cells via enhanced ALK1-Smad1/5 signaling and is the presumed isoform when not specified in the literature. In contrast, S-endoglin enhances ALK5-Smad2/3 signaling. Endoglin has specific receptor-ligand interactions between type I and type II TGF-β superfamily receptors when binding TGF-β superfamily ligands. Mouse CD105 has three separate regions of similarity to TGF-β receptor III but does not contain the RGD tripeptide found in human CD105.

CD105 (endoglin) is a TGF-β superfamily co-receptor that promotes angiogenesis, is involved in endothelial integrin-mediated mural cell and leukocyte adhesion, antagonizes TGF-β mediated ERK activation, is essential to the immune response of macrophages, regulates trophoblast differentiation and invasion during pregnancy, promotes T-cell proliferation, and regulates differentiation and collagen expression in myofibroblasts.

MJ7/18 was produced by immunizing rats with inflamed mouse skin and selecting for reactivity with endothelial cells. MJ7/18 predominantly stains vascular endothelial cells and is a marker of mouse endothelium

1.0 – 5.0 mg/ml

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and Azide free.

>95% by SDS-PAGE and HPLC

>98% by SDS-PAGE and HPLC

<1.0 EU/mg as determined by the LAL method

≤ 0.75 EU/mg as determined by the LAL method

Aggregation level ≤ 5%

Aggregation level ≤ 1%

Anti-Mouse CD105 In Vivo Antibody (MJ7/18) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

Agonist, Cell Separation, ELISA, Flow Cytometry, Immunofluorescence, IF Microscopy, Immunohistochemistry, IHC FF, Immunoprecipitation, LCI, Western Blot

Each investigator should determine their own optimal working dilution for specific applications.

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

Rat IgG2a In Vivo isotype control – Low Endotoxin [ICH2244]