Video of immunofluorescence staining anti-ICAM-1 antibody clone 15.2

With the help of our partner, Binaree – ichorbio has been creating new immunofluorescence validation data for a number of our antibodies.

One of these antibodies is anti-ICAM-1 antibody, clone 15.2. IF images can be found here, but we are lucky to be able to show today a 3D video of the antibody in action.

Immunofluorescence Staining Protocol:

Immunofluorescence analysis of paraffin-embedded breast cancer tissue section labeling ICAM-1 (1:100) overnight at 4˚, followed by goat anti-mouse IgG H&L (Alexa Fluor ® 647-red) secondary antibody (1:500 dilution). MDA-MB-231 cells were injected via mouse nipple and grown for 5 weeks. The nuclear counter stain is DAPI (blue).

Image was acquired on a Nikon A1R microscope system and 3D images were reconstructed with IMARIS software.

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