Rat Isotype Controls

• Rat immunoglobulins are critical components in immunological research, serving as isotype controls to assess non-specific binding and background noise in antibody-based assays.
• Isotype controls with rat immunoglobulins allow researchers to differentiate specific antibody binding from non-specific interactions, ensuring accurate interpretation of experimental results.
• Rat immunoglobulin isotype controls play a vital role in evaluating the specificity of antibody binding and establishing appropriate gating strategies in various immunological assays, including flow cytometry, immunohistochemistry, and ELISA.
• Selection of suitable rat immunoglobulin isotype controls depends on the specific class (e.g., IgG, IgM, IgA) and subclass (e.g., IgG1, IgG2a, IgG2b, IgG2c) of the test antibody.
• Matching the isotype control to the test antibody ensures comparable binding characteristics, including Fc receptor interactions and non-specific binding, allowing accurate assessment of background levels.