ichorGreen® Nucleic Acid Gel Stain (10,000x in water)
ichorGreen® Nucleic Acid Gel Stain is the third generation non-mutagenic fluorescent nucleic acid gel stain to replace the SYBR Green (SG) or the expensive GelGreen® (GG) from Biotium for staining dsDNA, ssDNA or RNA in agarose gels or polyacrylamide gels.
ichorGreen® is more sensitive than SG and GR either as precast gel stains or post gel stains.
We can directly replace SG or GG with ichorGreen® without changing the existing imaging system since they all have the similar spectra. ichorGreen® can also be used to stain dsDNA, ssDNA or RNA in polyacrylamide gel via post gel staining. Gel staining with ichorGreen® is compatible with downstream applications such as gel extraction and cloning. ichorGreen® is efficiently removed from DNA by phenol/chloroform extraction and ethanol precipitation.
Comparable Staining: better than GelGreen® or your money back
Safety: Nonmutagenic and noncytotoxic
Easy disposal: Safe to dispose in the drain
Compatibility: Spectrally compatible with existing instruments
Sensitivity: Higher signal but lower background
Stability: can be stored at RT and microwavable
Dilute ichorGreen® 10,000X stock reagent into the molten agarose gel solution at 1:10,000 (e.g. 5ul added to 50ml of the gel solution) and mix thoroughly. ichorGreen® can be added while the gel solution is still hot.
Cast the gel and allow it to solidify. Any leftover gel solution may be stored and reheated later for additional gel casting.
ichorGreen® precast gels may be stored at 4°C for later use.
Load samples and run the gels using your standard protocol.
Image the stained gel with a 254nm transiluminator, a Dark Reader or a similar transilluminator or a laser-based gel scanner using a long path green filter such as a SYBR filter or GelStar filter.
Dilute the ichorGreen® 10,000X stock reagent~3,300 fold to make a 3X staining solution in H2O. Note: including 0.1M NaCl in the staining solution enhances sensitivity, but may promote dye precipitation if the gel stain is reused.Carefully place the gel in a suitable polypropylene container. Gently add a sufficient amount of the 3X staining solution to submerge the gel.Agitate the gel gently at room temperature for 30 minutes.
ichorGreen: Image the stained gel with a 254nm transilluminator, a Dark Reader or a similar transilluminator, or a laser-based gel scanner using a long path green filter such as a SYBR filter or GelStar filter.
Staining solution can be resued 2-3 times. Store staining solution at room temperature protected from light