Bulk anti-Human PMEL Antibody (HMB-45) [ICH1187]

ichorbio’s bulk anti-human PMEL antibody (HMB-45) is manufactured in a cGMP compliant, ISO Quality Standard 9001:2015 facility.

PMEL

Melanoma Marker antibody, Human Melanoma Black, Melanocyte Protein PMEL

HMB-45

ichorbio’s anti-Human PMEL antibody is available in the following bulk sizes: 5mg, 25mg, 50mg and 100mg. ichorbio regularly manufactures multi-gram amounts of our anti-PMEL clone HMB-45 – please contact us for pricing.

Mouse IgG1 kappa

Mouse

Human

HMB-45 activity is directed against processed, mature human PMEL protein (also known as gp100, PMEL17, SILV), specifically sialylated PMEL in the fibrillar matrix of Stage II melanosomes. Additionally, HMB-45 stains melanoma and nevocytic lesions with high specificity by recognizing the melanosomal matrix fibers.

This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Cytoplasmic staining by HMB-45 is observed in 77-100% of primary melanomas and 56-83% of metastatic melanomas. HMB-45 also stains PEComas (i.e., lymphangiomyomatosis, angiomyolipomas, and pulmonary ‘sugar’ tumors), meningeal melanocytomas, sweat gland tumors, clear cell sarcoma of the tendons and aponeuroses as well as some ovarian steroid cell tumors, breast cancers, and renal cell carcinomas, but does not stain non-melanocytic tumors. HMB-45 has poor sensitivity to spindle cell and desmoplastic melanoma.

HMB-45 was generated in a BALB/c mouse using a portion of an axillary lymph node containing pigmented melanoma metastases as immunogen. The spleen was removed, fused with NS-1 cells, and the resulting hybridoma lines screened against the same melanoma used as immunogen. Deletion of PMEL’s internal proline/serine/threonine-rich repeat (RPT) domain abolishes recognition by HMB-45 as well as PMEL’s capacity to form fibrils.

This antibody was developed using an extract of pigmented melanoma metastases from lymph nodes.

>2.0 mg/ml

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (PBS) pH 7.2 – 7.4, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and azide free

>95% by SDS-PAGE and HPLC

>98% by SDS-PAGE and HPLC

≤ 1.0 EU/mg as determined by the LAL method

≤ 0.75 EU/mg as determined by the LAL method

Aggregation level ≤ 5%

Aggregation level ≤ 1%

This antibody is stable for at least 4 weeks when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 20°C or -80°C. Avoid repeated freeze thaw cycles.

Functional Assays, Flow Cytometry, Immunofluorescence Microscopy, IHC-Frozen, IHC-Paraffin, Western Blot

Each investigator should determine their own optimal working dilution for specific applications.

Products are for research use only. Not for use in diagnostic or therapeutic procedures.