Bulk anti-Human CD68 Antibody (KP1) [ICH1190]

ichorbio’s bulk anti-Human CD68 Antibody (KP1) is manufactured in a cGMP compliant, ISO Quality Standard 9001:2015 facility.

CD68

GP110, LAMP4, SCARD1, CD68 molecule

KP1

ichorbio’s anti-Human CD68 antibody is available in the following bulk sizes: 5mg, 25mg, 50mg and 100mg. ichorbio regularly manufactures multi-gram amounts of our anti-CD68 clone KP1 – please contact us for pricing.

Mouse IgG1 kappa

Mouse

Human

Anti-CD68 antibody (clone KP1) activity is directed against human CD68.

This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

CD68 is found in the cytoplasm of monocytes/macrophages, fibroblasts, human peripheral blood lymphocytes, neutrophil primary and mast cell granules, large granular lymphocytes, basophils, basal epithelial cell layers, renal glomeruli, myeloid cells, endothelial cells, retinal epithelial cells, osteoblasts, fibroblast-like cells from bone marrow, and a wide-range of lymphoid neoplasms. CD68 is predominantly located in lysosomal membranes, with a small amount on the cell surface. Additionally, CD68 can be expressed in most hematopoietic cell lines by phorbol-induced differentiation; soluble CD68 can also be found in serum and urine.

KP1 was originally used as a pan-monocytic/macrophage marker against CD68; however, CD68 is not a macrophage specific antigen and KP1 is known to stain neutrophils and other non-macrophage-like cells. KP1 reacts against CD68 in a wide range of healthy and disease-associated (rheumatoid arthritis and osteoarthritis)5 tissues as well as a variety of neoplasms, tumor cell lines, and tumor-associated macrophages. KP1 detects a fixation-resistant epitope that is likely glycan-based which is shared by many cell types. KP1 was developed by immunizing Balb/c mice against a lysosomal fraction of human lung . Hybridoma supernatants derived from spleen cells were screened on cryostat human lung and tonsil sections as well as paraffin wax sections of lung tissue fixed in formol saline.

Lysosomal fraction of human lung.

>2.0 mg/ml

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (PBS) pH 7.2 – 7.4, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and azide free

>95% by SDS-PAGE and HPLC

>98% by SDS-PAGE and HPLC

≤ 1.0 EU/mg as determined by the LAL method

≤ 0.75 EU/mg as determined by the LAL method

Aggregation level ≤ 5%

Aggregation level ≤ 1%

This antibody is stable for at least 4 weeks when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 20°C or -80°C. Avoid repeated freeze thaw cycles.

Functional Assays, Flow Cytometry, Immunofluorescence Microscopy, IHC-Frozen, IHC-Paraffin, Western Blot

Each investigator should determine their own optimal working dilution for specific applications.

Products are for research use only. Not for use in diagnostic or therapeutic procedures.