Bulk anti-human CD45RO antibody (UCHL-1)
ichorbio’s bulk anti-human CD45RO antibody (UCHL-1) is manufactured in a cGMP compliant, ISO Quality Standard 9001:2015 facility. ichorbio’s low endotoxin antibodies have half the endotoxin of comparable antibodies from our competitors at less than 1.0 EU/mg. If ichorbio’s low endotoxin antibodies are not low enough we also offer ultra low endotoxin antibodies which have even less endotoxin (<0.75EU/mg) at an even higher purity (98% versus 95%). ichorbio: the best antibodies for in vivo research.
CD45RO molecule is the 180 kDa isoform of CD45 Leucocyte Common Antigen (LCA)
ichorbio’s CD5 in vivo antibody is available in the following bulk sizes: 5mg, 25mg, 50mg and 100mg. ichorbio regularly manufactures multi-gram amounts of our anti CD45RO clone UCHL-1 – please contact us for pricing.
Mouse Anti-Human CD45 (Clone UCHL-1) recognizes Human CD45.
This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
The CD45RO antigen is present on most thymocytes, about 40% of resting peripheral blood T-lymphocytes and the majority of T-cells in skin reactive infiltrates and T-cell malignancies. CD45RO is also found on a subset of B-cells and some B-cell lymphomas. NK cells do not express the CD45RO antigen. It is also present on monocytes, macrophages and granulocytes.
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (PBS) pH 7.2 – 7.4, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and azide free
>95% by SDS-PAGE and HPLC
>98% by SDS-PAGE and HPLC
≤ 1.0 EU/mg as determined by the LAL method
≤ 0.75 EU/mg as determined by the LAL method
Aggregation level ≤ 5%
Aggregation level ≤ 1%
This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
Flow Cytometry, IHC (Frozen), IHC (Paraffin), Immunoprecipitation, Western Blot, CODEX®
Each investigator should determine their own optimal working dilution for specific applications.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.