Bulk anti-PD-L1 In Vivo Antibody – Low Endotoxin (10F.9G2)
ichorbio’s anti-PD-L1 In Vivo Antibody – Low Endotoxin (10F.9G2) is manufactured in a cGMP compliant facility. ichorbio’s low endotoxin antibodies have half the endotoxin of comparable antibodies from our competitors at less than 1.0 EU/mg. If ichorbio’s low endotoxin antibodies are not low enough we also offer ultra low endotoxin antibodies which have even less endotoxin (<0.75EU/mg) at an even higher purity (98% versus 95%). ichorbio: the best antibodies for in vivo research.
ichorbio’s 10F.9G2 in vivo antibody is available in the following bulk sizes:
1mg, 5mg, 25mg, 50mg and 100mg
ichorbio regularly manufactures multi-gram amounts of our anti-PD-L1 10F.9G2 clone – please contact us for pricing.
Programmed cell death 1 ligand 1, Cd274, PDCD1 ligand 1, Programmed death ligand 1, B7 homolog 1, B7-H1, B7h1, Pdcd1l1, Pdcd1lg1, Pdl1
Anti-PD-L1 In Vivo Antibody – Low Endotoxin (10F.9G2) recognizes an epitope on Mouse CD274
This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
The CD274 antigen is present on T cells, B cells, NK cells, dendritic cells, IFN-γ activated endothelial cells, and monocytes
CD274, also known as B7-H1 and PD-L1, is a 40 kD type I transmembrane protein and a member of the B7 family within the immunoglobulin receptor superfamily. It is expressed on T cells, B cells, NK cells, dendritic cells, IFN-γ activated endothelial cells, and monocytes.
1.0 – 5.0 mg/ml
0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and Azide free.
>95% by SDS-PAGE and HPLC
>98% by SDS-PAGE and HPLC
<1.0 EU/mg as determined by the LAL method
<0.5 EU/mg as determined by the LAL method
Aggregation level ≤ 5%
Aggregation level ≤ 1%
We use the IMPACT test generated by IDEXX Laboratories to guarantee our Ultra Low Endotoxin antibodies are pathogen free. Our rat antibodies are tested for:
Pneumonia virus of mice
Kilham’s rat virus
Toolan’s H1 virus
Lymphocytic choriomeningitis virus
Rat minute virus
anti-PD-L1 In Vivo Antibody – Low Endotoxin (10F.9G2) is stable when stored at 2-8°C. Do not freeze.
Flow Cytometry, Blocking, IHC (Frozen), Immunofluorescence, Functional Assays
Each investigator should determine their own optimal working dilution for specific applications.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
Sample: Frozen sections of tumor tissues from tumor bearing C57BL / 6 mice (inoculated with LLC cells)
1. Tumors were dissected, fixed in 4% paraformaldehyde, and dehydrated in 30% sucrose;
2. Frozen tumor sections were prepared at 25 ℃ and rinsed in PBS;
3. Blocking buffer: PBS containing 0.3% Triton + 5% goat serum; Sections were blocked for 1h;
4. Primary antibodies: Diluted in blocking buffer; incubated overnight at 4℃. Final concentration of ichorbio PD-L1 antibody clone 10F.9G2 (low) 1μg/ml, Final concentration (high) 5μg/ml. Positive signals were detected at both high and low concentrations
5. Washed by PBST; Secondary antibodies: incubated at 4℃ for 6h; DAPI: 2h
Details of secondary antibody:
Alexa Fluor 647-AffiniPure Goat Anti-Rat IgG (H+L) (min X Hu,Bov,Hrs Sr Prot) antibody – Jackson Immunoresearch Labs Cat# 112-605-062 – Conc. 7.5μg/ml.
6. Washed by PBST at least 6 times；
7. Add fluorescence decay resistant medium, seal slice;
8. Detected by the laser scanning confocal microscope.
Scale bar in the IF figure is 50 μM.
Images produced by Dr. Qin from State Key Laboratory of Genetic Engineering, School of Life Sciences of Fudan University