Anti-Mouse Ly49C In Vivo Antibody (4LO3311) – Low Endotoxin [ICH1202]

ichorbio’s anti-Mouse Ly49C In Vivo Antibody (4LO3311) – Low Endotoxin [ICH1202] is manufactured in a cGMP compliant, ISO Quality Standard 9001:2015 facility. ichorbio’s low endotoxin antibodies have half the endotoxin of comparable antibodies from our competitors at less than 1.0 EU/mg. If ichorbio’s low endotoxin antibodies are not low enough we also offer ultra low endotoxin antibodies which have even less endotoxin (<0.75EU/mg) at an even higher purity (98% versus 95%). ichorbio: the best antibodies for in vivo research.

Ly49C

4LO3311

ichorbio’s 4LO3311 in vivo antibody is available in the following bulk sizes: 5mg, 25mg, 50mg and 100mg. ichorbio regularly manufactures multi-gram amounts of our anti-mouse Ly49C LO3311 clone – please contact us for pricing.

Mouse IgG3 kappa

Ly-49 inhibitory receptors C, Killer cell lectin-like receptor 3, KLRA3; NK2.1

Mouse

Mouse

4LO3311 activity is directed against Ly49C. 4LO3311 binds an epitope located in a 32-amino acid segment of the stalk region immediately adjacent to the carbohydrate recognition domain.

Ly49C is expressed on NK, uterine NK, NKT, and CD8+ T cells.

4LO3311 recognizes an epitope located in a 32-amino acid segment of the stalk region immediately adjacent to the carbohydrate recognition domain. 4LO3311 recognizes Ly-49CBALB but not Ly-49AB6, Ly-49ABALB, BBALB, DB6, EB6, FB6, GB6, GBALB, or H. 4LO3311 plus complement inhibits NK cell and antibody-dependent cellular cytotoxicity (ADCC) activity in C3H spleen cells4. 4LO3311 inhibits C57BL/6 and C3H NK cell activity but has no effect on NZB NK cells.

4LO3311 was generated by immunizing 129/SvJ mice with C57BL/6 NK-enriched spleen cells.

1.0 – 5.0 mg/ml

0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added. BSA and Azide free.

>95% by SDS-PAGE and HPLC

>98% by SDS-PAGE and HPLC

<1.0 EU/mg as determined by the LAL method

≤ 0.75 EU/mg as determined by the LAL method

Aggregation level ≤ 5%

Aggregation level ≤ 1%

Anti-Mouse Ly49C In Vivo Antibody (4LO3311) is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

Depletion, Flow Cytometry, Immunoprecipitation

Each investigator should determine their own optimal working dilution for specific applications.

Products are for research use only. Not for use in diagnostic or therapeutic procedures.